FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12147101
Sequences flagged as poor quality0
Sequence length0-34
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG235524019.389317665177888No Hit
TAGCAGCACGTAAATATTGGCGT3715733.058943858291785No Hit
TGAGGTAGTAGATTGTATAGTT2924822.4078337703786277No Hit
ACAGATGATGAACTTATTG2305631.898090746096538No Hit
TGAGGTAGTAGTTTGTGCTGTT2237121.841690457665578No Hit
GGTTTCGTAAACCGAAGATC2183821.7978116753948121No Hit
TAGCAGCACGTAAATATTGGC1863911.5344484251839183No Hit
ATCACATTGCCAGGGATTTCC1752251.442525257672592No Hit
CAGCAGCAATTCATGTTTTGA1677021.3805927850604025No Hit
TAGCTTATCAGACTGATGTTGAC1665281.3709279275771231No Hit
TTCAAGTAATTCAGGATAGGTT1609061.324645279560942No Hit
TGAGGTAGTAGTTTGTACAGTT1594231.3124366052443295No Hit
TTCAAGTAATCCAGGATAGGCT1378111.1345176104158516No Hit
TCCCTACACGACGCTCTCCCTACACG1322161.0884572376569521No Hit
CACAGATGATGAACTTATTG1308871.0775163555485379No Hit
TGAGGTAGTAGGTTGTATAGTT781070.6430093896477851No Hit
ATCACATTGCCAGGGATTACC770920.6346534864573861No Hit
AGGGGTAAAGCACTGTTACGTAT759900.6255813629935241No Hit
TGAGGTAGTAAGTTGTATTGTT725200.5970148762243765No Hit
TAGCTTATCAGACTGATGTTGA667670.5496537815895332No Hit
ATCACATTGCCAGGGATTTCCA635910.5235076253996735No Hit
ATCACATTGCCAGGGATTTC625690.51509409529072No Hit
TAGCTTATCAGACTGATGTTG615490.5066970300156391No Hit
AACCGGGCGGAAACACCA611220.5031817879838161No Hit
ACCTCAGAAGGTCTCACTTTT593330.48845399408467916No Hit
TTCACAGTGGCTAAGTTCCG576440.4745494418791776No Hit
TAGCAGCACGTAAATATTGGCGA553890.45598534168769983No Hit
TCCCTACACGACGCTCTCCCTAC483970.39842428246871414No Hit
AAGCTGCCAGTTGAAGAACTGT444630.36603795424109836No Hit
TGGCTCAGTTCAGCAGGAACA344700.283771411796115No Hit
GGTATCGTAAACCGAAGATC341380.2810382493732455No Hit
TAGCAGCACGTAAATATTGG294480.24242821394174627No Hit
AAGCTGCCAGTTGAAGAACTG274800.22622681741100203No Hit
TTCAAGTAATCCAGGATAGGCTT273020.224761447196331No Hit
AACATTCAACGCTGTCGGTGAGT262780.21633145225350478No Hit
ACCATCGACCGTTGATTGTACC260870.21475906061866118No Hit
TGGCTCAGTTCAGCAGGAAC257030.21159781251510135No Hit
TGGCTCAGTTCAGCAGGAACAGT251720.2072263991218975No Hit
AGCTACATTGTCTGCTGGGTTT248590.20464965262081874No Hit
TTCAAGTAATCCAGGATAGGC242720.19981722387917908No Hit
TGAGGTAGTAGATTGTATAGTTT241720.1989939821855437No Hit
TAGCAGCACGTAAATATTGGCGTT235770.19409569410841318No Hit
TGTAAACATCCCCGACTGGAAGCT233960.19260562664293315No Hit
TAGGGGTAAAGCACTGTTACGTAT233610.19231749205016077No Hit
TCCCTAC232130.1910990943435804No Hit
TAGCACCATCTGAAATCGGTT226490.1864560111914769No Hit
TTTGGCAATGGTAGAACTCACACT225010.1852376134848965No Hit
TTTGGCACTAGCACATTTTTGCT216140.1779354596623507No Hit
TAGCAGCACGTAAATATTGGCA215470.17738388772761501No Hit
ATCACATTGCCAGGGATTACCACT194900.16044980608953527No Hit
GGTGTCGTAAACCGAAGATC193980.15969242373139073No Hit
GGCTGTAGGAATACTTTT191880.15796361617475643No Hit
TAGGTAGTTTCCTGTTGTTGGG189130.15569970151725912No Hit
TGTAAACATCCCCGACTGGAAGC186710.15370745661866153No Hit
AGGCAAGATGCTGGCATAGCT182290.15006872833279317No Hit
TGAGGTAGTAGATTGTATAGT163100.13427072023193024No Hit
AGCTCGGTCTGAGGCCCCTCAGT155690.12817049928209207No Hit
ACTGGACTTGGAGTCAGAAGGC155070.12766008943203816No Hit
TGGCAGCACGTAAATATTGGCG154920.12753660317799284No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC153630.1264746213932032No Hit
AGCAGCATTGTACAGGGCTATGT153590.12644169172545777No Hit
TCCCACATGGTCTAGCGGTTAGGATT150680.12404605839697884No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA150060.12353564854692489No Hit
AGGCAAGATGCTGGCATAGC149330.12293468211057107No Hit
TACCAGCACGTAAATATTGGCG149080.12272887168716223No Hit
AGAGGTAGTAGGTTGCATAGTT146930.12095890204584615No Hit
GCCGAAATAGCTCTCCCTACACG146660.1207366267885646No Hit
AGCTACATTGTCTGCTGGGTTTC144740.11915600273678469No Hit
CAGTGGTTTTACCCTATGGTAG141220.11625819197518816No Hit
TGGCTCAGTTCAGCAGGA140880.11597828979935211No Hit
AACCCGTAGATCCGAACTTGTG139200.11459524375404467No Hit
ACATATGATGAACTTATTG138260.11382139656202744No Hit
ACCATCGACCGTTGATTGTAC137470.11317103562405548No Hit
ATATAATACAACCTGCTAAGTG136760.11258653402157436No Hit
AACATTCATTGCTGTCGGTGGGTT136300.11220784284250208No Hit
TAGCGGCACGTAAATATTGGCG136200.11212551867313857No Hit
AGCTACATTGTCTGCTGGGTT128770.10600883288942768No Hit
AAGGAGCTCACAGTCTATTGAG128080.10544079612081927No Hit
TAGGTAGTTTCCTGTTGTTGGGA126810.10439527916990235No Hit
TCGAGGAGCTCACAGTCTAGT125040.10293814137216774No Hit
TCGAGGAGCTCACAGTCTAGTA123600.10175267333333278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACATA57.3916494E-43327.288627
CCGGGCT15000.02972.377426
CATCATA59.953704E-42867.39925
CGGGCTA17200.02766.292227
GGGCTAA20150.02569.2128
GGACTAA1150.02525.707828
TCCGGGC4800.02299.892825
CGGACTA3200.02189.101628
CGAGCTA500.02050.280328
GGGCAAA1000.01830.008526
AATTACG50.0027112861737.499422
CGACGGA2300.01683.038325
GCCTATA100.00295606861663.644327
GCCACAA100.00295606861663.644326
GCCAATA600.01663.644226
ACGGACT4250.01644.071827
TCGCCAC950.01618.642628
TCGCCTA800.01494.996228
CCAATAT600.01386.370127
CCGCCAC750.01366.853628
Produced by FastQC (version 0.11.5)