FastQCFastQC Report
Wed 27 Apr 2022
EGAF00002441089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11989976
Sequences flagged as poor quality0
Sequence length0-34
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG211116817.60777502807345No Hit
TAGCAGCACGTAAATATTGGCGT3720183.102741823670039No Hit
TAGCTTATCAGACTGATGTTGAC3499642.918804841644387No Hit
TTCAAGTAATCCAGGATAGGCT2843432.371505998010338No Hit
TGAGGTAGTAGTTTGTGCTGTT2466922.057485352764676No Hit
GGTTTCGTAAACCGAAGATC2007841.6745988482378946No Hit
TGAGGTAGTAGATTGTATAGTT1990061.659769794368229No Hit
TGAGGTAGTAGTTTGTACAGTT1944461.6217380251636868No Hit
TCCCTACACGACGCTCTCCCTACACG1483511.2372918844875085No Hit
TAGCAGCACGTAAATATTGGC1290631.0764241729925064No Hit
TTCAAGTAATTCAGGATAGGTT1116950.9315698380046799No Hit
AACCGGGCGGAAACACCA1001780.8355145998624185No Hit
TCCCTACACGACGCTCTCCCTAC800610.6677327794484326No Hit
TAGCTTATCAGACTGATGTTGA624930.5212103844077753No Hit
ACAGATGATGAACTTATTG612920.5111936837905263No Hit
AACCCGTAGATCCGAACTTGTG597690.4984914064882199No Hit
TAGCTTATCAGACTGATGTTG583820.48692341002183825No Hit
ATCACATTGCCAGGGATTTCC583030.48626452630097006No Hit
TGAGGTAGTAGGTTGTATAGTT510530.4257973493858537No Hit
AGGGGTAAAGCACTGTTACGTAT445550.371602078269381No Hit
TGAGGTAGTAAGTTGTATTGTT438790.36596403529081295No Hit
ACCTCAGAAGGTCTCACTTTT438070.36536353367179386No Hit
TCCCTAC428920.3577321589300929No Hit
ATCACATTGCCAGGGATTACC413300.3447046099174844No Hit
CACAGATGATGAACTTATTG407580.33993395816638833No Hit
CTTCGTGATCGATGTGGTGACGTC403320.33638099025385876No Hit
TAGCAGCACGTAAATATTGGCGA396360.33057614127000756No Hit
AACCCGTAGATCCGAACTTGT380940.3177153982626821No Hit
CTTCGTGATCGATGTGGTGACG356580.297398426819203No Hit
AACCCGTAGATCCGATCTTGT327410.2730697709486658No Hit
TTCGTGATCGATGTGGTGACG309640.2582490573792642No Hit
TTTGGCAATGGTAGAACTCACACT308640.25741502735284877No Hit
TTCAAGTAATCCAGGATAGGC308350.2571731586451883No Hit
GGTATCGTAAACCGAAGATC305210.2545543043622439No Hit
TTCGTGATCGATGTGGTGACGTC299740.24999216011775172No Hit
TAGGTAGTTTCCTGTTGTTGGG285400.23803216953895487No Hit
AACCCGTAGATCCGATCTTGTG270030.22521312803295018No Hit
TTCGTGATCGATGTGGTGACGTCG266280.22208551543389243No Hit
GTTTCCGTAGTGTAGTGGTTATC265140.22113472120377892No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA264380.22050085838370317No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT261110.21777358019732485No Hit
TTTGGCACTAGCACATTTTTGCT256600.21401210477819138No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC252550.210634283171209No Hit
AGCTCGGTCTGAGGCCCCTCAGT249140.2077902407811325No Hit
GCCCGGATAGCTCAGTCGGTAGAGC243420.20301958903003642No Hit
AAGCTGCCAGTTGAAGAACTGT237420.19801540887154404No Hit
AATGACACGATCACTCCCGTTGAGT204340.17042569559772264No Hit
TGTAAACATCCCCGACTGGAAGCT201050.16768173681081597No Hit
TTCAAGTAATCCAGGATAGGCTT188510.15722300027956687No Hit
ATCACATTGCCAGGGATTTCCA184400.15379513687099958No Hit
TCCCACATGGTCTAGCGGTTAGGATT179920.1500586823526586No Hit
CTGACCTATGAATTGACAGCC171300.14286934352495786No Hit
GGCTTGGTCTAGGGGTATGATT166510.1388743396984281No Hit
AATCCCGAGTGTTGTTCTC166430.13880761729631486No Hit
GGTGTCGTAAACCGAAGATC166010.1384573246852204No Hit
GAATCCCGAGTGTTGTTCTC165950.13840728288363546No Hit
GCCCGGATAGCTCAGTCGG164890.13752321105563514No Hit
TAGGTAGTTTCCTGTTGTTGGGA164070.13683930643397452No Hit
ACACGACGCTCTTCCGATCTCAC163960.1367475631310688No Hit
GAAAATGTTTAG161820.13496273887453986No Hit
TAGCACCATCTGAAATCGGTT160770.1340870073468037No Hit
ACTGGACTTGGAGTCAGAAGGC159270.13283596230718062No Hit
CACCCGTAGAACCGACCTTGCG159000.13261077420004844No Hit
TGGCTCAGTTCAGCAGGAACA157370.13125130525699136No Hit
AGTAAGGTCAGCTAAATAAGCTA155010.12928299439465102No Hit
TGTAAACATCCTCGACTGGAAGCT153100.1276899970441976No Hit
TAGGGGTAAAGCACTGTTACGTAT153070.1276649761434051No Hit
GCCGAAATAGCTCTCCCTACACG150960.12590517278766863No Hit
TACCAGCACGTAAATATTGGCG148350.12372835441872443No Hit
TATGGCTTTTCATTCCTATGTGA145000.12093435383023286No Hit
CAACGGAATCCCAAAAGCAGCT143000.11926629377740205No Hit
TGGCAGCACGTAAATATTGGCG142770.11907446687132651No Hit
TCAGTGCACTACAGAACTTTGT140470.1171561978105711No Hit
TTCACAGTGGCTAAGTTCTGC139870.11665577979472186No Hit
TAGCAGCACATCATGGTTTACA139380.11624710508177831No Hit
GGCTGTAGGAATACTTTT135390.11291932527638088No Hit
ATATAATACAACCTGCTAAGTG133520.11135968912698409No Hit
TAGCGGCACGTAAATATTGGCG129820.10827377802924709No Hit
TAGCAGCACGTAAATATTGG127180.10607193875951043No Hit
TGGCTCAGTTCAGCAGGAAC126510.10551313864181212No Hit
TAGCTTATCAGACTGATGTTGACA124710.10401188459426441No Hit
TGTAAACATCCCCGACTGGAAGC123500.10300270826230178No Hit
CAACGGAATCCCAAAAGCAGCTG122680.10231880364064115No Hit
ATCCCGAGTGTTGTTCTC122100.10183506622532022No Hit
GCCCGGATAGCTCAGTCGGTAGA120140.10020036737354604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)