FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13923776
Sequences flagged as poor quality0
Sequence length0-34
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG275144919.760796209304143No Hit
TCCCTACACGACGCTCTCCCTACACG12536889.003936863103803No Hit
TCCCTACACGACGCTCTCCCTAC5378423.862759642211998No Hit
TAGCAGCACGTAAATATTGGCGT3923432.817791668007299No Hit
ACACGACGCTCTTCCGATCTCAC2467391.7720695880198016No Hit
GGTTTCGTAAACCGAAGATC1824071.3100397478385173No Hit
TAGCTTATCAGACTGATGTTGAC1747601.2551193009712307No Hit
TAGCAGCACGTAAATATTGGC1744151.2526415248277478No Hit
ACCTCAGAAGGTCTCACTTTT1349900.969492758286258No Hit
TGAGGTAGTAGTTTGTGCTGTT1186660.852254445920417No Hit
TTCAAGTAATTCAGGATAGGTT1156990.8309455710864638No Hit
TTCAAGTAATCCAGGATAGGCT1085210.7793934633823468No Hit
TCCCTAC1077390.7737771707904523No Hit
GGTTCCATAGTGTAGCGGTTATC1069160.7678664178452741No Hit
CCTACACGACGCTCTCCCTACACG918860.6599215615074532No Hit
AACCCGTAGATCCGAACTTGTG876950.6298219678340129No Hit
CACGACGCTCTTCCGATCTCC778660.5592304846041763No Hit
GCCGAAATAGCTCTCCCTACACG775980.5573057193680795No Hit
AACCCGTAGATCCGAACTTGT655720.4709354703781503No Hit
TACACGACGCTCTTCCGATCTCTAC636960.45746211372547213Illumina Single End PCR Primer 1 (100% over 21bp)
TGAGGTAGTAGTTTGTACAGTT626560.4499928754958425No Hit
CACAGATGATGAACTTATTG602830.43295008480458175No Hit
ATCACATTGCCAGGGATTACC596760.4285906351840191No Hit
ATCACATTGCCAGGGATTTCC569770.40920652558616283No Hit
AACCCGTAGATCCGATCTTGT546170.39225710037277245No Hit
CAGTGCAATGTTAAAAGGGCATT509670.36604294697070683No Hit
TGAGGTAGTAGATTGTATAGTT503920.3619133200649019No Hit
TAGCAGCACGTAAATATTGGCGA458620.3293790420069958No Hit
CAGCAGCAATTCATGTTTTGA445740.3201286777379929No Hit
GCCCGGATAGCTCAGTCGGTAGAGC444460.31920938687896155No Hit
AACCCGTAGATCCGATCTTGTG400300.2874938522423802No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA395950.2843696997136409No Hit
ACAGATGATGAACTTATTG387540.2783296714914115No Hit
CTTCGTGATCGATGTGGTGACG374810.26918703661995136No Hit
CCCGTACGGGCCACCA361070.2593190238050368No Hit
AACCGGGCGGAAACACCA353610.25396128176724475No Hit
CCCCCGCGGGGGCGCGCCGGCG340290.24439491126544985No Hit
ACGACGCTCTTCCGATCTC331290.2379311474128857No Hit
TACACGACGCTCTTCCGATCCCTAC324330.23293250336690277No Hit
TAGCTTATCAGACTGATGTTG313720.22531244398071326No Hit
TAGCTTATCAGACTGATGTTGA306830.22036407365358365No Hit
AGTAAGGTCAGCTAAATAAGCTA305220.21920777811995826No Hit
TCCCACATGGTCTAGCGGTTAGGATT304430.21864040329289983No Hit
TTCGTGATCGATGTGGTGACG296450.21290919934362634No Hit
CCTACACGACGCTCTCCCTAC289360.20781718981977304No Hit
ATCACATTGCCAGGGATTACCACT284330.2042046640221733No Hit
GGTATCGTAAACCGAAGATC270920.19457365588185274No Hit
TCCGTGATAGTTTAATGGTCAGA238000.1709306441011404No Hit
GCCCGGATAGCTCAGTCGG237620.17065772962736545No Hit
AATCCCGAGTGTTGTTCTC237100.17028426771588395No Hit
TCCCTACACGACGCTCCTCCCTACACG236570.1699036238445663No Hit
TTTGGCACTAGCACATTTTTGCT229340.16471106688300646No Hit
CCCTACACGACGCTCTCCCTAC225480.16193883038624005No Hit
ACCATCGACCGTTGATTGTACC225070.1616443700329566No Hit
CCTAC224460.16120627048294944No Hit
GTTTCCGTAGTGTAGTGGTTATC223760.1607035332944167No Hit
TGAGGTAGTAGGTTGTATAGTT223360.1604162549009694No Hit
CTTCGTGATCGATGTGGTGACGTC222850.1600499749493241No Hit
AGGCAAGATGCTGGCATAGCT220490.15835503242798504No Hit
CCCGCGAGGGGGGCCCGGGCACC219050.1573208302115748No Hit
GTATGCCGTCTTCTGCTTGAAAAAAAAATATTTA215650.1548789638672728No Hit
CGACGCTCTTCCGATCTCCG209190.15023941781309896No Hit
CACGTCGGGGTCACCA207180.14879584388602632No Hit
AACCCGTAGATCCGATCTTGTGT207050.14870247840815595No Hit
AGGCAAGATGCTGGCATAGC193890.13925101926373995No Hit
GAATCCCGAGTGTTGTTCTC192510.13825990880634678No Hit
TAGGTAGTTTCCTGTTGTTGGG190040.13648596472680974No Hit
TTCGTGATCGATGTGGTGACGTC179220.1287150841840604No Hit
ATCCCGAGTGTTGTTCTC177220.12727869221682395No Hit
TCCCTACACGACGCTCCTCCCTAC175110.12576329869138947No Hit
ATCACATTGCCAGGGATTTCCA174450.12528928934220143No Hit
CCTTCGATAGCTCTCCCTACACG169180.12150439650853331No Hit
TTCAAGTAATCCAGGATAGGCTT167690.12043428449294215No Hit
TCCCATATGGTCTAGCGGTTAGGATT163600.11749686291994356No Hit
AGGCAAGATGCTGGCATAGCTG161560.11603174311336235No Hit
GTTTCCGTAGTGTAGTGGTCATC158220.11363296852807743No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC157440.1130727756608552No Hit
AAAGGTTCGTTTGTAAAAAA157270.1129506823436401No Hit
TAGCTTATCAGACTGATGTTGACC149740.1075426665869948No Hit
AAAGGTTCGTTTGTAAAAA149200.10715484075584095No Hit
AGCTCGGTCTGAGGCCCCTCAGT147110.10565381115007882No Hit
TAGCAGCACGTAAATATTGGCGTT143730.10322630872544919No Hit
AACCCGTAGATCCGAACTTGTGT143450.10302521385003609No Hit
TAGCACCATCTGAAATCGGTT143060.10274511741642496No Hit
TGGCTCAGTTCAGCAGGAACAGT142740.10251529470166713No Hit
AAGCTGCCAGTTGAAGAACTGT141700.10176837087870418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA201.0913936E-111666.195925
ATATTTA41700.01596.518328
ATAATAT6300.01507.510625
TACACAG550.01470.183527
AATATTT45600.01458.003727
GACGGCA252.1827873E-111437.512726
CGGCTAA9200.01425.79428
ACAAATA5450.01420.60524
TTTACGC50.00424524681388.582923
GACCTAA201.6692866E-81347.668326
CGACGGA1700.01323.155425
AAAATAT29000.01321.465625
AACAAAT5350.01284.763523
AAATTTA10700.01284.693128
ATAATTA1200.01272.797728
GACGGGA1750.01232.153926
ACGAGGG900.01216.522724
ACGGCTA10600.01212.053827
TCGCCAC1200.01197.927228
ACGACGG17000.01186.876224
Produced by FastQC (version 0.11.5)