FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13952517
Sequences flagged as poor quality0
Sequence length0-34
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG217063615.557307688641412No Hit
TGAGGTAGTAGTTTGTGCTGTT8960096.421844890065355No Hit
TGAGGTAGTAGATTGTATAGTT5763674.130917740505172No Hit
TAGCTTATCAGACTGATGTTGAC4534053.249628722903545No Hit
TAGCAGCACGTAAATATTGGCGT4048292.901476486285593No Hit
TTCAAGTAATCCAGGATAGGCT2911162.0864765834006866No Hit
TTCAAGTAATTCAGGATAGGTT2650141.899399226677165No Hit
AACCCGTAGATCCGAACTTGTG1915321.3727415634039364No Hit
TGAGGTAGTAGTTTGTACAGTT1879231.3468752627214144No Hit
TGAGGTAGTAGGTTGTATAGTT1450521.0396117059022398No Hit
AACCCGTAGATCCGAACTTGT1398841.0025717940354417No Hit
ATCACATTGCCAGGGATTACC1339740.9602138452868397No Hit
TAGCAGCACGTAAATATTGGC1319180.9454781527949402No Hit
GGTTTCGTAAACCGAAGATC1174590.8418481052558473No Hit
ATCACATTGCCAGGGATTTCC1081430.7750787904433301No Hit
TGAGGTAGTAAGTTGTATTGTT1079350.7735880199966788No Hit
TAGCTTATCAGACTGATGTTGA1049940.7525093859409023No Hit
TAGCTTATCAGACTGATGTTG814670.5838874806602995No Hit
ACAGATGATGAACTTATTG751250.5384333163686523No Hit
AGCTACATTGTCTGCTGGGTTTC727060.521095942760722No Hit
TCCCTACACGACGCTCTCCCTACACG620720.44488030367567366No Hit
AGGGGTAAAGCACTGTTACGTAT589200.4222893976764192No Hit
ACCTCAGAAGGTCTCACTTTT498350.35717569811955796No Hit
CACAGATGATGAACTTATTG436910.313140632618473No Hit
AACCGGGCGGAAACACCA434050.3110908232543275No Hit
TTCAAGTAATCCAGGATAGGCTT419210.30045474949071915No Hit
AGCTACATTGTCTGCTGGGTTT403960.2895248219371458No Hit
TTCAAGTAATCCAGGATAGGC400250.2868658034962437No Hit
AAAGGTTCGTTTGTAAAAAA387720.2778853449882913No Hit
TAGCAGCACGTAAATATTGGCGA385240.276107887917284No Hit
TAGCTTATCAGACTGATGTTGACC375100.2688403819898589No Hit
ATCACATTGCCAGGGATTTCCA365300.2618165596931364No Hit
AAAGGTTCGTTTGTAAAAA361620.259179042749061No Hit
CAAAGTGCTGTTCGTGCAGGTAG336660.24128979738924525No Hit
AAAGGTTCGTTTGTAAAAAAA330950.23719734582656307No Hit
TAAAGGTTCGTTTGTAAAAA325850.23354209136602377No Hit
TGGCTCAGTTCAGCAGGAACA325320.23316223158875204No Hit
TTTGGCAATGGTAGAACTCACACT304030.21790333600740283No Hit
TCGAGGAGCTCACAGTCTAGT298270.21377504861667612No Hit
TGGCTCAGTTCAGCAGGAAC296620.21259246629120754No Hit
AAGGAGCTCACAGTCTATTGAG294590.21113753167260074No Hit
TAAAGTGCTGACAGTGCAGAT286520.205353629026218No Hit
AACCCGTAGATCCGAACTTGTGT280500.20103899532965988No Hit
TCGAGGAGCTCACAGTCTAGTA271570.19463871644091169No Hit
TAAAGGTTCGTTTGTAAAAAA270400.19380015806467035No Hit
TTCACAGTGGCTAAGTTCTGC268870.19270358172650856No Hit
TGAGGTAGTAGATTGTATAGTTT259990.1863391386658049No Hit
CTGACCTATGAATTGACAGCC258090.18497737720011379No Hit
AAAGGTTCGTTTGTAAAAAAAA253900.18197433480998448No Hit
AACCCGTAGATCCGATCTTGT251010.1799030239490122No Hit
ATCACATTGCCAGGGATTACCACT249880.17909313423520645No Hit
TCCCTACACGACGCTCTCCCTAC243000.1741621242962829No Hit
TGAGGTAGTAGATTGTATAGT241340.1729723748052054No Hit
TAAAGTGCTGACAGTGCAGATA239030.17131675954954936No Hit
TAGCTTATCAGACTGATGTTGACA234950.16839255598111796No Hit
TAGCACCATCTGAAATCGGTT233140.16709529900590694No Hit
ATCACATTGCCAGGGATTTC231580.16597722117091848No Hit
AAGCTGCCAGTTGAAGAACTGT229420.16442911339939595No Hit
TAAAGGTTCGTTTGTAAAAAAA228240.16358338785754573No Hit
AGCTCGGTCTGAGGCCCCTCAGT223280.16002847371553103No Hit
AGAGGTAGTAGGTTGCATAGTT221030.15841586145352843No Hit
TTCCCCGACGGGGAGCC215440.15440941587815302No Hit
ACTGGACTTGGAGTCAGAAGGC204890.14684805616076296No Hit
TGTAAACATCCCCGACTGGAAGCT202760.14532144988606716No Hit
TTAGGTAGTAGTTTGTGCTGTT201930.1447265751405284No Hit
TTTGGCACTAGCACATTTTTGCT196390.1407559653931975No Hit
TGGCTCAGTTCAGCAGGA194250.1392221919528928No Hit
AACCCGTAGATCCGATCTTGTG193750.13886383367244778No Hit
ATATAATACAACCTGCTAAGTG193290.13853414405443834No Hit
GGTATCGTAAACCGAAGATC187450.13434851933884046No Hit
TAGGGGTAAAGCACTGTTACGTAT186700.1338109819181729No Hit
AGCTACATTGTCTGCTGGGTT185040.1326212324270954No Hit
TGTAACAGCAACTCCATGTGGA181340.12996938115180223No Hit
TTCACAGTGGCTAAGTTCCG181040.1297543661835352No Hit
AAGGTTCGTTTGTAAAAAAA179630.12874379583268022No Hit
TAAAGGTTCGTTTGTAAAAAAAA177890.12749670901673155No Hit
AGTAAGGTCAGCTAAATAAGCTA174850.12531789067162577No Hit
TGTAACAGCAACTCCATGTGG173620.12443632930173101No Hit
GGCTCAGTAGCCAGTGTAGATCC172580.12369094407840536No Hit
TGAGGTAGTAGTTTGTGCTGT168910.12106059429993887No Hit
TGGCTCAGTTCAGCAGGAACAGT168510.12077390767558285No Hit
TGTAAACATCCCCGACTGGAAGC165500.11861659082730378No Hit
AAGGTTCGTTTGTAAAAAA164870.11816505939394305No Hit
TTCAAGTAATCCAGGATAGG162670.11658828295998494No Hit
CATTGCACTTGTCTCGGTCTGA161280.11559204694034776No Hit
AAAGGTTCGTTTGTAAAAAAAAA160620.11511901401016031No Hit
TGGCAGCACGTAAATATTGGCG158820.11382892420055822No Hit
TGAGGTAGTAGGTTGTATAGT158490.1135924077354645No Hit
TAGGATGGGGTGTGATAGGTGGC152920.10960029649130691No Hit
TAAAGGTTCGTTTGTAAAA152880.10957162782887131No Hit
TAGCGGCACGTAAATATTGGCG151370.10848938582192731No Hit
AGCAGCATTGTACAGGGCTATGT147400.10564402107519381No Hit
AACCCGTAGATCCGAACTTGTGA146220.10479829553334355No Hit
AGCAGCATTGTACAGGGCTATGA146220.10479829553334355No Hit
TAGCAGCACATCATGGTTTACA145760.10446860591533412No Hit
TAGCAGCACGTAAATATTGGCA144520.10357987737983046No Hit
AAGGTTCGTTTGTAAAAAAAA143970.10318568327134094No Hit
TCCCTAC142260.10196009795221893No Hit
TAGCACCATTTGAAATCAGTGTT140800.10091369177331946No Hit
TATTAAAGGTTCGTTTGTAAAAA140550.10073451263309695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)