FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002442547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002442547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49204
Sequences flagged as poor quality0
Sequence length239
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT27135.513779367531095No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT20344.133810259328509No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT18943.849280546297049No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC16713.3960653605397932No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT16043.2598975693033085No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC15973.2456710836517355No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC12772.5953174538655395No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC11982.434761401512072No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC11962.4306966913259087No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC11762.390049589464271No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT10972.229493537110804No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC10362.1055198764328105No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT10242.081131615315828No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC9621.9551255995447523No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC8591.7457930249573206No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC8111.648239980489391No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT8091.6441752703032275No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT8001.6258840744654908No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT7761.5771075522315259No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT7561.5364604503698887No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC6541.3291602308755386No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6381.2966425493862288No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC6321.2844484188277376No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC6251.2702219331761644No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC5451.1076335257296155No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC5451.1076335257296155No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT4991.0141451914478496No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC4890.9938216405170311No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC4670.9491098284692301No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC4600.9348833428176572No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC4480.9104950817006747No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC3880.7885537761157629No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3760.7641655149987806No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT3680.7479066742541257No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC3360.682871311275506No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT3030.6158035932038046No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2910.5914153320868222No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2820.5731241362490855No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT2660.5406064547597756No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC2580.5243476140151206No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC2460.4999593528981384No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2200.4471181204780099No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC2180.4430534102918462No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT2170.44102105519876433No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC2140.43492398991951875No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC2120.43085927973335497No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC2100.4267945695471913No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC2050.41663279408178194No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1950.39630924315096333No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC1740.35362978619624424No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC1660.3373709454515893No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT1650.33533859035850744No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1640.3333062352654256No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC1630.3312738801723437No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC1610.32720916998618No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1600.32517681489309813No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1580.3211121047069344No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1530.3109503292415251No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1500.30485326396227946No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1460.2967238435899521No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1460.2967238435899521No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1460.2967238435899521No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1390.282497357938379No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1300.26420616210064224No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC1290.2621738070075603No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC1280.2601414519144785No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT1240.252012031542151No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1200.2438826111698236No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT1200.2438826111698236No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1190.2418502560767417No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT1180.23981790098365988No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1160.23575319079749613No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1160.23575319079749613No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1130.22965612551825057No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1050.21339728477359565No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1040.21136492968051376No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1030.2093325745874319No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1030.2093325745874319No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT980.19917079912202257No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC970.19713844402894073No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT970.19713844402894073No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT910.18494431347044954No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGAATGAT900.18291195837736768No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC880.17884724819120398No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT880.17884724819120398No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT870.17681489309812212No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC860.17478253800504023No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT800.16258840744654907No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC760.1544589870742216No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGAATTAC760.1544589870742216No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC760.1544589870742216No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT750.15242663198113973No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT740.1503942768880579No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT730.14836192179497604No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC720.14632956670189415No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC710.1442972116088123No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT700.14226485651573043No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT690.14023250142264856No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC680.1382001463295667No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT680.1382001463295667No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC680.1382001463295667No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT660.13413543614340298No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC650.13210308105032112No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGAATGAT640.13007072595723926No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC640.13007072595723926No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC620.1260060157710755No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT620.1260060157710755No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGAATGAT620.1260060157710755No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGAATTAC610.12397366067799366No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGAATTAC610.12397366067799366No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC610.12397366067799366No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT600.1219413055849118No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC580.11787659539874806No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT580.11787659539874806No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC580.11787659539874806No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT570.1158442403056662No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC530.10771481993333874No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGAATTAC530.10771481993333874No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC510.10365010974717503No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT500.10161775465409317No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC500.10161775465409317No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT500.10161775465409317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)