FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002442680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002442680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33312408
Sequences flagged as poor quality0
Sequence length42-76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1374100.41248894405952286RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG677280.2033116309094197RNA PCR Primer, Index 29 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407540.1223387994047143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)