FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002442682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002442682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13173743
Sequences flagged as poor quality0
Sequence length39-76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG317280.24084271265956836RNA PCR Primer, Index 29 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270750.2055224547799361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174450.13242250133466243No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG160950.12217484430962408RNA PCR Primer, Index 29 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152910.11607179523693456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)