FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002442690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002442690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17343089
Sequences flagged as poor quality0
Sequence length32-76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391360.22565760920675665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241830.13943882776591873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216770.12498926805945584No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG184160.10618638928739858RNA PCR Primer, Index 29 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)