FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002443531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002443531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49613
Sequences flagged as poor quality0
Sequence length5-68180
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTC159.1121066E-4287.55512
TGTTATC750.0258.79962
CTAATGT351.5011574E-7246.475781
TAATGTC604.0017767E-11215.666322
TTGTCCG1500.0186.910831
TTGTCTC851.8189894E-12177.607571
AATGTCC651.6176273E-8166.233353
TTGTTAT1500.0158.15531
CGATGTC300.0072080935144.06893
GTTATCC753.8022336E-8144.06893
TTGATAG300.0072518922143.777561
CTAATCC602.2012573E-6143.777561
TTGGTAT1051.2732926E-11143.777541
TTGTTGC1400.0138.642621
TATCGTC959.586074E-10136.763465
TCGTCTC552.8123625E-4117.636182
TGGTATC756.6791017E-6115.022042
TTGTCGT951.5770456E-7113.50861
TTTAGTT603.89632E-4108.3810968
CGTCTCC809.112762E-6108.0516743
Produced by FastQC (version 0.11.5)