FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002443612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002443612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27636
Sequences flagged as poor quality0
Sequence length5-69884
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT370.13388334057027065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTAG102.824483E-4424.781286
TACTCGT102.8400982E-4424.001835
TCGTGTA102.9031342E-4420.91261
CGGTACT159.6742244E-4281.634372
TGTTATC251.31258E-5253.470932
TTGGTAT700.0240.521471
TTGTTAT452.077286E-9233.84031
TACTTTA302.6771448E-5212.000935
GGTACTT200.002289106211.22583
CCCGTAT200.0023142933210.45631
TTGGTAC355.0973256E-5180.39111
CAAATTG250.0043179253170.85478
CGTTAGT250.0043900777169.91256
GCTTGTT250.0044143056169.600745
TATCCGT250.0044143056169.600745
GTACTTT250.004438622169.290124
CCGTATC250.004463027168.980622
CTTGCGC250.004463027168.980623
GTTATCC250.004463027168.980623
TCGGTAC250.004512105168.365041
Produced by FastQC (version 0.11.5)