FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002444423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002444423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20648985
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT511200.24756664794903963No Hit
CTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAA449090.21748768765147536No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338750.1640516470906439No Hit
CAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTCCCAGG282040.13658782744042866No Hit
GTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGC251780.12193335410917291No Hit
GTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCC227510.11017974975525431No Hit
AGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCAGTTCACTCAGCTGGGC224250.10860097966074363No Hit
GCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCC224240.10859613680769296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)