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PL:ILLUMINA PU:170403_HS31_22207_A_CAKHRANXX_8#116 LB:18808290 DS:EGAS00001001755: In this study we will compare the single cell transcriptomes of immune cells from asthmatics responding to steroids, those refractory to steroids, and a control population. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ DT:2017-04-03T01:00:00+0100 PG:SCS SM:EGAN00001510138 CN:SC @PG ID:SCS PN:RTA VN:1.18.66.3 DS:Controlling software on instrument @PG ID:basecalling PN:RTA PP:SCS VN:1.18.66.3 DS:Basecalling Package @PG ID:bambi PN:bambi PP:basecalling VN:0.9.4 DS:Convert Illumina BCL to BAM or SAM file CL:/software/solexa/pkg/bambi/0.9.4/bin/bambi i2b --intensity-dir=/nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities --basecalls-dir=/nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BaseCalls --lane=8 --platform-unit=170403_HS31_22207_A_CAKHRANXX_8 --read-group-id=22207_8 --study-name="EGAS00001001755: In this study we will compare the single cell transcriptomes of immune cells from asthmatics responding to steroids, those refractory to steroids, and a control population. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/" 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--output-file=- --compression-level=0 @PG ID:bamadapterfind PN:bamadapterfind PP:bambi VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamadapterfind level=0 @PG ID:bambi.1 PN:bambi PP:bamadapterfind VN:0.9.4 CL:/software/solexa/pkg/bambi/0.9.4/bin/bambi decode --metrics-file /nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/22207_8.bam.tag_decode.metrics --barcode-file /nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/metadata_cache_22207/lane_8.taglist --compression-level 0 - @PG ID:bamcollate2 PN:bamcollate2 PP:bambi.1 VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamcollate2 collate=2 level=0 @PG ID:bwa PN:bwa PP:bamcollate2 VN:0.7.15-r1140 CL:/software/solexa/pkg/bwa/0.7.15/bwa sampe /lustre/scratch117/core/sciops_repository/references/PhiX/Sanger-SNPs/all/bwa0_6/phix_unsnipped_short_no_N.fa /tmp/SAbthjA9LK/alnphix_bwa_aln_1_out /tmp/FB1X3BDXwQ/alnphix_bwa_aln_2_out /tmp/BGc26FFDqP/alnphix_simple_cat1_out /tmp/jDQNFrrKek/alnphix_simple_cat2_out @PG ID:scramble PN:scramble PP:bwa VN:1.14.9 CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -t 1 -I sam -O bam @PG ID:bam12auxmerge PN:bam12auxmerge PP:scramble VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=1 zztoname=0 clipreinsert=1 /tmp/6kmVqqhpFg/simple_cat_out @PG ID:scramble.1 PN:scramble PP:bam12auxmerge VN:1.14.9 CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I bam -O cram -x -3 @PG ID:scramble.2 PN:scramble PP:scramble.1 VN:1.14.9 CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I cram -O bam -0 @PG ID:spf PN:spatial_filter PP:scramble.2 DS:A program to apply a spatial filter VN:v10.27-dirty CL:/software/solexa/pkg/pb_calibration/10.27/bin/spatial_filter -c -F /dev/stdout -t /nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/no_cal/archive/qc/tileviz/22207_8 --region_size 200 --region_mismatch_threshold 0.0160 --region_insertion_threshold 0.0160 --region_deletion_threshold 0.0160 /dev/stdin ; /software/solexa/pkg/pb_calibration/10.27/bin/spatial_filter -a -f -u -l /nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/no_cal/22207_8.bam.filter.stats -F /tmp/h4p_CroaSE/tee_post_filter_creation:__APPLY_FILTER_OUT___out /dev/stdin @PG ID:samtools PN:samtools PP:spf VN:1.4 CL:/software/solexa/pkg/samtools/1.4/bin/samtools split -f /nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/no_cal/lane8/%!.bam - @PG ID:bamcollate2' PN:bamcollate2 PP:samtools VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamcollate2 collate=1 level=0 @PG ID:bamreset PN:bamreset PP:bamcollate2' VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamreset resetaux=0 level=0 verbose=0 @PG ID:bamadapterclip PN:bamadapterclip PP:bamreset VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamadapterclip verbose=0 level=0 @PG ID:bwa' PN:bwa PP:bamadapterclip VN:0.7.15-r1140 CL:/software/solexa/pkg/bwa/0.7.15/bwa mem -t 16 -p -Y -K 100000000 /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/GRCh38_15/all/bwa0_6/Homo_sapiens.GRCh38_15.fa /tmp/MvKjcgadux/alntgt_bamtofastq_out @PG ID:scramble' PN:scramble PP:bwa' VN:1.14.9 CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -I sam -O bam @PG ID:samtools' PN:samtools PP:scramble' VN:1.4 CL:/software/solexa/pkg/samtools/1.4/bin/samtools reheader /tmp/66vCvwCyd3/postalntgt_alterSQ_headerSQfix_out /tmp/t5iDT2jCFN/postalntgt_mbuffer_headerSQfix_out @PG ID:bam12split PN:bam12split PP:samtools' VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bam12split verbose=0 level=0 @PG ID:bamsormadup PN:bamsormadup PP:bam12split VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamsormadup threads=16 SO=queryname level=0 @PG ID:bam12auxmerge' PN:bam12auxmerge PP:bamsormadup VN:2.0.72 CL:/software/solexa/pkg/biobambam/2.0.72/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=0 zztoname=0 clipreinsert=1 /tmp/vcKhX3rOel/amp_bamadapterclip_pre_auxmerge_out @PG ID:AlignmentFilter PN:AlignmentFilter PP:bam12auxmerge' DS:Give a list of SAM/BAM files with the same set of records and in the same order but aligned with different references, split reads into different files according to alignments. You have option to put unaligned reads into one of output files or a separate file VN:V1.19 CL:uk.ac.sanger.npg.picard.AlignmentFilter INPUT_ALIGNMENT=[./initial_phix_aln_22207_8#116.bam, /tmp/dhJSv76Csw/postalntgt_bam12auxmerge_out] OUTPUT_ALIGNMENT=[/tmp/yYBthaa4Ng/alignment_filter:__PHIX_BAM_OUT___out, /dev/stdout] METRICS_FILE=22207_8#116.bam_alignment_filter_metrics.json VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=SILENT COMPRESSION_LEVEL=0 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false @PG ID:bamsormadup' PN:bamsormadup CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamsormadup threads=16 SO=coordinate level=0 verbose=0 fixmate=1 adddupmarksupport=1 tmpfile=/nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/no_cal/archive/lane8/bsfopt_22207_8#116.tmp PP:AlignmentFilter VN:2.0.72 @PG ID:bamstreamingmarkduplicates PN:bamstreamingmarkduplicates CL:/software/solexa/pkg/biobambam/2.0.72/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/no_cal/archive/lane8/bmdfopt_22207_8#116.tmp M=/nfs/sf44/ILorHSany_sf44/analysis/170403_HS31_22207_A_CAKHRANXX/Data/Intensities/BAM_basecalls_20170406-044929/no_cal/archive/lane8/22207_8#116.markdups_metrics.txt PP:bamsormadup' VN:2.0.72 @PG ID:scramble.3 PN:scramble PP:bamstreamingmarkduplicates VN:1.14.9 CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -t 7 -7 -I bam -O cram -r /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/GRCh38_15/all/fasta/Homo_sapiens.GRCh38_15.fa @CO TY:checksum ST:all PA:all HA:crc32prod CO:441962 BS:21061ffd NS:28874e78 SQ:539e4d6e ST:BC,FI,QT,RT,TC:1f7053f1 @CO TY:checksum ST:all PA:pass HA:crc32prod CO:441886 BS:4a0f9d77 NS:75febe8c SQ:6a1cf812 ST:BC,FI,QT,RT,TC:660aae53 @CO TY:checksum ST: PA:all HA:crc32prod CO:0 BS:1 NS:1 SQ:1 ST:BC,FI,QT,RT,TC:1 @CO TY:checksum ST: PA:pass HA:crc32prod CO:0 BS:1 NS:1 SQ:1 ST:BC,FI,QT,RT,TC:1 @CO TY:checksum ST:22207_8#116 PA:all HA:crc32prod CO:441962 BS:21061ffd NS:28874e78 SQ:539e4d6e ST:BC,FI,QT,RT,TC:1f7053f1 @CO TY:checksum ST:22207_8#116 PA:pass HA:crc32prod CO:441886 BS:4a0f9d77 NS:75febe8c SQ:6a1cf812 ST:BC,FI,QT,RT,TC:660aae53