FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002446380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002446380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38154
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA511313.400954028411174No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA439411.516485820621691No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA35449.288672223095874No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG26236.874770666247314No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA15934.175184777480736No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA14523.8056298160088065No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG13183.454421554751795No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA10622.7834565183204907No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA9212.413901556848561No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG6031.5804371756565498No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6011.57519526130943No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA5531.4493893169785605No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4981.3052366724327724No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTTGCA3981.043140955076794No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA3280.8596739529276091No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG3260.8544320385804896No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA3190.8360853383655711No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3180.8334643811920114No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG2500.655239293389946No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1610.42197410494312526No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1500.3931435760339676No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1420.3721759186454893No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1310.3433453897363317No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1190.3118939036536143No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1180.3092729464800545No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTCATCGTCCAGCA1150.30141007495937516No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1130.2961681606122556No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG1070.2804424175708969No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA1060.2778214603973371No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG870.22802327409970122No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA860.22540231692614143No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA860.22540231692614143No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA840.22016040257902186No Hit
CCTGTTTGATACACACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA770.20181370236410337No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA620.16249934476070663No Hit
CCTGTTTGCTCCACACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA610.15987838758714684No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGTG600.15725743041358703No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCTAGCA590.15463647324002724No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGTA560.14677360171934792No Hit
CCTGTTTGCTCACCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA550.1441526445457881No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA540.14153168737222832No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA530.13891073019866856No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGTA510.133668815851549No Hit
CCTGTTTGATACACACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG480.12580594433086964No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGAGTCAGTTGCAGTCCAGTG470.12318498715730986No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA450.11794307281019027No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA440.1153221156366305No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG390.10221732976883158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)