FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002446414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002446414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42163
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA496211.768612290396794No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG34208.111377273913147No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG19654.660484310888694No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG8151.9329744088418754No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA7891.8713089675782086No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4591.088632213077817No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA4401.0435690060005216No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA4331.02696677181415No Hit
CCTGTTTGATCCCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG4150.9842753124777649No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA3940.934468609918649No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3700.8775466641368024No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA3230.7660745203140193No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG3120.7399852951640064No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2580.6119109171548515No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG2220.5265279984820814No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG2110.5004387733320683No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA2110.5004387733320683No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1980.4696060527002348No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA1690.4008253682138368No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1530.36287740435927235No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1490.3533904133956312No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCCAGCA1440.34153167469107987No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1210.2869814766501435No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1070.2537770082773996No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1060.2514052605364893No Hit
CCTGATTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1040.24666176505466877No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1030.2442900173137585No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA940.222944287645566No Hit
CCTGTTTGATCCCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG910.21582904442283518No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA900.21345729668192492No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTCATGTCCCAGAG890.21108554894101467No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCCAGTG880.20871380120010435No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA840.19922681023646327No Hit
CCTGATTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG770.1826245760500913No Hit
CCTGTTTGATCCCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG760.18025282830918102No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCACGAGCGTCAGTCATGTCCCAGAG760.18025282830918102No Hit
CCTGTTTGATCCCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG730.1731375850864502No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA720.17076583734553993No Hit
CCTGTTTGATCCCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA690.1636505941228091No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCACTGTCAGTTGCAGCTTAGCA650.154163603159168No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA650.154163603159168No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA610.14467661219552688No Hit
CCTGTTAGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG590.13993311671370634No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTGACAGTCTAGTG580.13756136897279606No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA580.13756136897279606No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCCAGCA570.13518962123188577No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGATGCAGCTTAGCA560.13281787349097549No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTATCGTCCAGTA540.12807437800915494No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCCTAGCA530.12570263026824466No Hit
CCTGTTTGATACCCACACTTACGAGCCTCAATGTCAGTTGCAGCTTAGCA520.12333088252733439No Hit
CCTGTTTGACACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG520.12333088252733439No Hit
CCTGATTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG500.11858738704551383No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCCTAGCA480.11384389156369329No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTACAGTCTAGTG460.10910039608187275No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTACAGTCCAGTG460.10910039608187275No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA450.10672864834096246No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA440.10435690060005218No Hit
CCTGTTAGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA430.1019851528591419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)