FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002446582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002446582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34651
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA787022.712187238463535No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTCTAGCGTCAGTATCTGTCCAGTA22946.620299558454301No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG18695.393783729185305No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGCATCTGTCCAGTA12423.58431214106375No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG9452.7271940203745926No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA9312.686791145998672No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG8902.5684684424691926No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA6771.9537675680355544No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA5981.7257799197714352No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA5841.6853770453955152No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA4641.3390666936019162No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTCTAGCGTCAGCATCTGTCCAGTA3821.1024212865429568No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGT3450.9956422614065973No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATTAGCGTCAGTATCTGTCCAGTA2350.6781911055957981No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGTG2140.6175867940319183No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTTTCTGTCCAGTA1860.5367810452800785No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1680.48483449251103866No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1610.46463305532307875No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA1340.3867132261695189No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1270.36651178898155895No Hit
CCTGTTTGCTACCCACGCTTTCGCGCACTAGCGTCAGTATCTGTCCAGTA1200.34631035179359904No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTCTAGCGTCAGAATCTGTCCAGTA1200.34631035179359904No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG1050.30302155781939916No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTTAGCGTCAGTATCTGTCCAGTA1040.30013563822111916No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGAATCTGTCCAGTA970.2799342010331592No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA900.2597327638451993No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT890.2568468442469193No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA860.24818908545207932No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA860.24818908545207932No Hit
CCTTTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA770.2222158090675594No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCCAGTG720.2077862110761594No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA710.20490029147787941No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA680.19624253268303946No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA660.19047069348647946No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTCATCGTCCAGCA620.1789270150933595No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA610.1760410954950795No Hit
CCTTTTTGCTACCCACGCTTTCGCGCTCTAGCGTCAGTATCTGTCCAGTA550.15872557790539954No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG540.15583965830711954No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA540.15583965830711954No Hit
CCCGTTTGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA530.15295373870883958No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA530.15295373870883958No Hit
CCTGTTTGCTACCCACGCTTACGCGCTCTAGCGTCAGTATCTGTCCAGTA520.15006781911055958No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTATCTGTCCAGTA520.15006781911055958No Hit
CCTGTTTGCTACCCACACTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA510.14718189951227958No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCCGTA490.1414100603157196No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG480.13852414071743963No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA440.12698046232431964No Hit
CCTGTTTGCCACCCACGCTTTCGCGCTCTAGCGTCAGTATCTGTCCAGTA400.11543678393119967No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA400.11543678393119967No Hit
CCTGTTTGCTACCCACGCTTACGCGCTTTAGCGTCAGTATCTGTCCAGTA390.11255086433291969No Hit
CCTGTTTGCTACCCACGCTCTCGCGCTCTAGCGTCAGTATCTGTCCAGTA380.1096649447346397No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG370.10677902513635969No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTAACAGTCTAGTG360.1038931055380797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)