FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002446588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002446588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41030
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA27296.651230806726785No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA24135.881062637094809No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGTTTAGCA17314.218864245673897No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGCA16183.943456007799171No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA15843.86058981233244No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGCA14453.5218133073360955No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTTAGCA10872.649281013892274No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA9762.3787472581038265No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA8812.147209359005606No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA8722.1252741896173535No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA7151.742627345844504No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA6921.6865708018523031No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG5631.3721667072873507No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA4771.1625639775773824No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4581.1162563977577382No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTTCA2860.6970509383378015No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA2630.6409943943456008No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTATCA2360.5751888861808433No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTACA2340.570314404094565No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1820.4435778698513283No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGTA1690.4118937362905192No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1630.3972702900316842No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGTA1600.38995856690226666No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1590.38752132585912746No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAACA1390.33877650499634415No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCCTAGCA1300.31684133560809163No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAACGTCAGTTACAGCTTAGCA1300.31684133560809163No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTCTAGCA1270.3095296124786741No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA1250.30465513039239583No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGCT1210.2949061662198391No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA1100.2680965147453083No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1050.25591030952961247No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA1020.248598586400195No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA860.20960272970996832No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA840.20472824762369No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTTAGCA830.20229100658055083No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAACGTCAGTCGCGGTTTAGCA820.19985376553741163No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTCAGCA700.17060687301974164No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTTGCC680.1657323909334633No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCGGTTTAGCA680.1657323909334633No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAACGTCAGTGGCGGTTTTGCA650.15842066780404582No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCCTAGCA640.15598342676090665No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTCCAGCA630.15354618571776749No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGCT590.14379722154521082No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTCAGCA580.14135998050207166No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAATCCAGTA580.14135998050207166No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG580.14135998050207166No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC570.1389227394589325No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGCC560.13648549841579333No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAACGTCAGTCGCGGCTTAGCA560.13648549841579333No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA560.13648549841579333No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTA520.12673653424323666No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA520.12673653424323666No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGTA510.1242992932000975No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAACGTCAGTGGCGGTTTAGCA500.12186205215695832No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA480.11698757007068No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA470.11455032902754082No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCCCAGCA460.11211308798440166No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTCTTGCA420.102364123811845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)