FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002446604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002446604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48604
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA796716.391655007818287No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA29055.976874331330754No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA23514.8370504485227555No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA21124.3453213727265245No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG16933.4832524072092834No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG16723.4400460867418317No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA14522.9874084437494854No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG10092.075960826269443No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA7331.5081063286972265No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA6821.4031766932762737No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA6771.3928894741173565No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA5311.0925026746769813No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA4750.9772858200971114No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA4720.9711134886017612No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA4550.9361369434614434No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTTGCA4050.8332647518722739No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3860.7941733190683895No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA3710.7633116615916385No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA3530.7262776726195375No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA3190.6563245823389021No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTCTAGCGTCAGTATCTGTCCAGTA2730.5616821660768662No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA1950.40120154719776147No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACTGTCCAGTA1610.3312484569171261No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCCAGCA1610.3312484569171261No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG1500.30861657476750887No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1420.2921570241132417No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1270.2612953666364908No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCTAGTG1230.25306559130935724No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1230.25306559130935724No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA1100.22631882149617316No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG970.19957205168298903No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA960.19751460785120567No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA930.1913422763558555No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA920.1892848325240721No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACACTCCAGTG860.17694016953337174No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA830.17076783803802156No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA820.16871039420623818No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA790.162538062710888No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCACTGTCAGTTGCAGCTTAGCA780.16048061887910461No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA660.1357912928977039No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA640.1316764052341371No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTTTCTGTCCAGTA620.12756151757057033No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTCATCGTCCAGCA620.12756151757057033No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA610.12550407373878694No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA600.12344662990700354No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA600.12344662990700354No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA590.12138918607522015No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCCTAGCA580.11933174224343676No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA570.11727429841165335No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA530.1090445230845198No Hit
CCTGTTTGATACCCACACTTACGAGCCTCAATGTCAGTTGCAGCTTAGCA520.1069870792527364No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTTAGCA500.1028721915891696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)