FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002446700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002446700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52318
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG635012.137314117512139No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG49979.551206085859551No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG35526.789250353606789No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA32626.234947819106234No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA29165.573607553805574No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG22714.340762261554341No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA17983.4366757139034365No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA11032.108261019152108No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA10472.0012232883520014No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG8711.664818991551665No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG7141.3647310677013647No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA6781.295921097901296No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA6621.2653388891012654No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG5301.013035666501013No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG4660.8907068313008908No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG3990.7626438319507626No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3470.6632516533506633No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG3390.647960548950648No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGTG2920.5581253106005581No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2840.5428342062005429No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA2450.4682900722504683No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2420.46255590810046254No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA2380.45491035590045487No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2050.3918345502503918No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1950.3727206697503727No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1760.3364042968003364No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA1710.3268473565503268No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTAACAGTCTAGTG1690.323024580450323No Hit
CCTGTTTGATCCCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1660.3172904163003173No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGT1490.2847968194502848No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG1480.2828854314002829No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1470.280974043350281No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1460.27906265530027907No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA1320.2523032226002523No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1250.23892350625023892No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG1190.22745517795022743No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCCAGTG1150.2198096257502198No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCTAGTG1120.21407546160021407No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1000.19113880500019115No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA910.17393631255017392No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA910.17393631255017392No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA830.15864520815015865No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGAGTCAGTTGCAGTCCAGTG830.15864520815015865No Hit
CCTGTTTGCTCCCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG830.15864520815015865No Hit
CCTGTTTGATCCCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG820.15673382010015674No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA800.1529110440001529No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTACAGTCTAGTG710.13570855155013573No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG680.12997438740012998No Hit
CCTGTTTGCTACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG630.12041744715012041No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG630.12041744715012041No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA600.11468328300011468No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTAACAGTCCAGTG580.11086050690011086No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCTAGTG550.10512634275010513No Hit
CCTGTTTGATCCCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA530.1013035666501013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)