FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47808
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA16913.5370649263721554No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA8791.8386044176706826No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA6991.462098393574297No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA6981.4600066934404283No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA6341.3261378848728247No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA5981.2508366800535475No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA5961.2466532797858099No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA5951.2445615796519411No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA5891.2320113788487284No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA5321.1127844712182062No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA4530.9475401606425703No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA3230.6756191432396251No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA3110.6505187416331994No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA3030.633785140562249No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG2970.6212349397590361No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTNAGCA2670.5584839357429718No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA2590.5417503346720214No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNNAGAA2580.5396586345381527No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNNAGCA2530.5292001338688086No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA2470.5166499330655957No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA2430.5082831325301205No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA2380.49782463186077647No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA2350.4915495314591701No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2300.48109103078982596No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2270.47481593038821956No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA2260.47272423025435073No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2220.46435742971887545No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2210.4622657295850067No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2120.4434404283801874No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCNAGAA2120.4434404283801874No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA2100.4392570281124498No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA2080.4350736278447122No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2060.43089022757697454No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2060.43089022757697454No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2050.4287985274431058No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2030.42461512717536815No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGAA2030.42461512717536815No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA2010.42043172690763053No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA1960.40997322623828647No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA1930.40369812583668No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1920.4016064257028112No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1880.393239625167336No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA1870.3911479250334672No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1850.38696452476572957No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1750.3660475234270415No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1710.35768072289156627No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1620.338855421686747No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG1590.3325803212851406No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA1570.328396921017403No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA1540.32212182061579653No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1430.2991131191432396No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1410.294929718875502No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG1390.2907463186077644No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1380.28865461847389556No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTTTCAGTCCAGAA1320.2761044176706827No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA1280.2677376171352075No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1190.24891231593038823No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1150.24054551539491298No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1140.2384538152610442No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA1130.23636211512717537No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA1110.23217871485943772No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1100.23008701472556894No Hit
CCTGTTTGATACCNACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA1080.2259036144578313No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1080.2259036144578313No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1060.2217202141900937No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA1050.2196285140562249No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGNTTCAGTCCAGAA1020.2133534136546185No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1020.2133534136546185No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1010.21126171352074966No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA1010.21126171352074966No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1010.21126171352074966No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA990.20707831325301207No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA970.20289491298527443No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA960.2008032128514056No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG960.2008032128514056No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA930.19452811244979917No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA930.19452811244979917No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA930.19452811244979917No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA920.1924364123159304No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA910.1903447121820616No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG900.18825301204819278No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAACGTCAGTTACAGTNTAGCA900.18825301204819278No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTTTCGTCCAGAA900.18825301204819278No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA900.18825301204819278No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGTG890.18616131191432395No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGTG890.18616131191432395No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA890.18616131191432395No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA880.18406961178045514No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA870.18197791164658636No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG870.18197791164658636No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA870.18197791164658636No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA860.17988621151271755No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGNTACAGTTTAGCA860.17988621151271755No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA860.17988621151271755No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA860.17988621151271755No Hit
CCTGTTTGATACCCACACTTTNGAGCATGAACGTCAGTTACAGTTTAGCA850.17779451137884872No Hit
CCTGTTTGCTCCCCACGCTTTNGTGCCTCAGTGTCAGTTTCAGTCCAGAA850.17779451137884872No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA850.17779451137884872No Hit
CCTGTTTGATACCNACACTTTCGAGCNTGAANGTNAGTTACNGTNTAGCA850.17779451137884872No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA840.1757028112449799No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA830.1736111111111111No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCNCAGTA800.16733601070950468No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA800.16733601070950468No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA790.16524431057563588No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA790.16524431057563588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCNAGTA780.16315261044176707No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGAA780.16315261044176707No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA770.16106091030789826No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG760.15896921017402946No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTA760.15896921017402946No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA760.15896921017402946No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA740.15478580990629182No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTTTCAGTCCAGAA730.152694109772423No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGAA720.15060240963855423No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA720.15060240963855423No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA720.15060240963855423No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA720.15060240963855423No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA710.14851070950468542No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTAATCGTNCAGTA710.14851070950468542No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA710.14851070950468542No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCTAGTG710.14851070950468542No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCNNAGTA700.1464190093708166No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAANGTCAGTTACAGTNNAGCA690.14432730923694778No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCNTCAGNGTNAGTTTCNGTNCAGAA690.14432730923694778No Hit
CCTGTTTGANACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA680.14223560910307897No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTTTNGTNCAGAA680.14223560910307897No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTA670.1401439089692102No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTNAGTGTCAGTTTCAGTNCAGAA670.1401439089692102No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA670.1401439089692102No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA660.13805220883534136No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA650.13596050870147255No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA640.13386880856760375No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA630.13177710843373494No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA630.13177710843373494No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA620.12968540829986613No Hit
CCTGTTTGCNCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGAA620.12968540829986613No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA610.12759370816599733No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGNGTCAGTTTCAGTNNAGAA610.12759370816599733No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNAATCGTCCAGTA600.12550200803212852No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA600.12550200803212852No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTAATCGTNNAGTA590.1234103078982597No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCNTAGCA590.1234103078982597No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA570.1192269076305221No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA550.11504350736278447No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA550.11504350736278447No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA540.11295180722891565No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTAATNGTNCAGTA540.11295180722891565No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA530.11086010709504684No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA530.11086010709504684No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA530.11086010709504684No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTAATCGTCCAGTA520.10876840696117805No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTGCAGTNNAGTG510.10667670682730925No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA490.10249330655957162No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACTGTCCAGTA480.1004016064257028No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA480.1004016064257028No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGTG480.1004016064257028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)