FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22316
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA19048.531994981179423No Hit
TAGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCCATGCCGCGTGT18428.254167413514967No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA16297.299695285893529No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA13626.1032443090159525No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGC10934.8978311525362965No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA10324.62448467467288No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA9514.261516400788672No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT7423.3249686323713923No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG7063.1636493995339667No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA6973.12331959132461No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA5922.6528051622154507No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA5362.4018641333572326No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA5222.339128876142678No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA5082.276393618928123No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA4441.9896038716615883No Hit
TGGGGGATATTGGACAATGGGGGCAACCCTGATCCAGCGACGCCGCGTGA4321.9358307940491128No Hit
TAGGGAGTATTTGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGT3631.6266355977773796No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGC3291.4742785445420328No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTCGCGTGA3191.4294676465316365No Hit
TAGGGAATTTTCGTCAATGGGGGGAACCCTGAACGAGCAATGCCGCGTGA2951.321921491306686No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTAGCGTGC2901.2995160423014878No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA2581.15612116866822No Hit
TGAGGAATATTGGTCAATGGACGAAAGTCTGAACCAGCCAAGTAGCGTGC2341.0485750134432694No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGA2261.0127262950349525No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGC2220.9948019358307941No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC1760.788671804982972No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA1730.7752285355798529No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA1700.7617852661767341No Hit
TGGGGAATCTTGCGCAATGGGGGGAACCCTGACGCAGCGACGCCGCGTGC1600.7169743681663381No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGATGCCGCGTGG1580.7080121885642587No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC1390.6228714823445062No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC1120.5018820577164366No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG1080.4839576985122782No Hit
TGGGGAATCTTGCGCAATGGGCGGAAGCCTGACGCAGCGACGCCGCGTGC990.44362789030292166No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA940.4212224412977236No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA930.416741351496684No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA800.35848718408316904No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA720.3226384656748521No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA720.3226384656748521No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA680.30471410647069364No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAATGCCGCGTGA670.3002330166696541No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCTTGAACCAGCCAAGTAGCGTGC630.2823086574654956No Hit
TGGGGAATTTTGGACAATGGGGGAAACCCTGATCCAGCCATGCCGCGTGC590.2643842982613372No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA570.2554221186592579No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGA530.23749775945509946No Hit
TGGGGAATATTGGGCAATGGAGGGAACTCTGACCCAGCAACGCCGCGTGA490.21957340025094102No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAACGCCGCGTGA480.21509231044990143No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCTGCCAAGTAGCGTGC470.2106112206488618No Hit
TGGGGAATCTTCCGCAATGGGCGCAAGCCTGACGGAGCAACGCCGCGTGA460.2061301308478222No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAATGCCGCGTGA450.20164904104678255No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA430.19268686144470337No Hit
TGGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCAACGCCGCGTGA410.18372468184262414No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGTGCGACGCCGCGTGG400.17924359204158452No Hit
TGGGGGATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA400.17924359204158452No Hit
TCGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCAACGCCGCGTGA390.1747625022405449No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC370.16580032263846567No Hit
TAGGGAATGTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA370.16580032263846567No Hit
TAGGGAATTTTCGGCAATGGACGGAAGTCTGACCGAGCAACGCCGCGTGA360.16131923283742605No Hit
TTAGGAATATTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGA360.16131923283742605No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGGGACGCCGCGTAT340.15235705323534682No Hit
TGGGGAATTTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGC330.14787596343430723No Hit
TTAGGAATATTCGTCAATGGGGGGAACCCTGAACGAGCAATGCCGCGTGA320.1433948736332676No Hit
TGGGGAATATTGGGCAATGGACGAAAGTCTGACCCAGCGACGCCGCGTGA310.138913783832228No Hit
TAGGGAATGTTCGTCAATGGGGGGAACCCTGAACGAGCAATGCCGCGTGA300.13443269403118838No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGA280.12547051442910914No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA270.12098942462806955No Hit
TGGGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGC250.11202724502599032No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGA250.11202724502599032No Hit
TTAGGAATATTCGGCAATGGGGGAAACCCTGACCGAGCAATGCCGCGTGT240.10754615522495072No Hit
TGGGGAATTTTGGACAATGGGCGAAAGCCTGATCCAGCAACGATGCGTGG230.1030650654239111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)