FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28892
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9453.2708016059808944No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA9433.2638792745396645No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA5201.7998061747196457No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA3731.2910148137892843No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA3551.2287138308182197No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA3531.22179149937699No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA3291.1387235220822374No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA3221.1144953620379345No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA2991.0348885504637961No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA2800.9691264017721168No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG2780.9622040703308875No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCCCGCC2530.8756749273155199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2250.7787622871383082No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2220.768378789976464No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2160.7476117956527759No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTTAGCA2010.6956943098435553No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTTAGCA1940.6714661497992525No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA1900.6576214869167936No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA1750.605704001107573No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA1740.6022428353869583No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA1330.46033504084175547No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA1300.4499515436799114No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATCAGCGTCAGTGTGCAACTGGCA1280.443029212238682No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1260.4361068807974526No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA1260.4361068807974526No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1260.4361068807974526No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1250.43264571507683786No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1150.39803405787069085No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1140.3945728921500761No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1120.38765056070884674No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1120.38765056070884674No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1110.384189394988232No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG1110.384189394988232No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1100.3807282292676173No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA1100.3807282292676173No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1090.3772670635470026No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1080.37380589782638796No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA1070.37034473210577323No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1070.37034473210577323No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNNGGTA1060.3668835663851585No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1060.3668835663851585No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1040.3599612349439291No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1040.3599612349439291No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1040.3599612349439291No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1020.35303890350269973No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNCCGCC1000.3461165720614703No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA990.34265540634085556No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA990.34265540634085556No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTNAGTG960.3322719091790115No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNNAGTG960.3322719091790115No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA950.3288107434583968No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCNGGTA880.3045825834140939No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA840.29073792053163505No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA820.28381558909040566No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA810.28035442336979094No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA810.28035442336979094No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA800.2768932576491762No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCNTAGCA800.2768932576491762No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTGCGGCA800.2768932576491762No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA780.2699709262079468No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA780.2699709262079468No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA750.2595874290461027No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNNCGCC730.2526650976048733No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTNAGCA720.24920393188425863No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACAGTCCAGTA710.2457427661636439No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCNCGCC700.2422816004430292No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCNTAGCA680.2353592690017998No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTNAGTTGCAGCTTAGCA670.23189810328118513No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTNAGCA630.2180534403987263No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCNNAGCA630.2180534403987263No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA630.2180534403987263No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACAGTNCAGTA620.2145922746781116No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACAGTNNAGTA610.21113110895749687No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCNNAGCA590.20420877751626745No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA580.20074761179565276No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA580.20074761179565276No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA580.20074761179565276No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACAGTCCAGTA580.20074761179565276No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA570.19728644607503806No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGTTACGCTCCCGCC570.19728644607503806No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA560.19382528035442337No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA550.19036411463380865No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTCATGGCACGGAC540.18690294891319398No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA540.18690294891319398No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACGGCTTAGTG540.18690294891319398No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT540.18690294891319398No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA530.18344178319257926No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA530.18344178319257926No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA520.17998061747196456No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATCAGCGTCAGTGTGCAANNGGCA500.17305828603073514No Hit
CCTGTTCGATACCNGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNCCGCC500.17305828603073514No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA480.16613595458950575No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGNGTCAGTTGCGCTNNGGTA480.16613595458950575No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA480.16613595458950575No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTNAGCA470.16267478886889106No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA470.16267478886889106No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA460.15921362314827633No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTNAGTCGCGGCTTAGCA460.15921362314827633No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA450.15575245742766164No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA450.15575245742766164No Hit
CCTGTTTGATACCNACACTTTCGAGCNTGAANGTNAGTTACNGCNTAGTG440.15229129170704694No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA430.14883012598643225No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATCAGCGTCAGTGTGCAANTGGCA430.14883012598643225No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA430.14883012598643225No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA430.14883012598643225No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNNAGCA420.14536896026581753No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAANGTCAGTTACGGCNNAGTG420.14536896026581753No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA420.14536896026581753No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA410.14190779454520283No Hit
CNTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA410.14190779454520283No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCNTAGCA400.1384466288245881No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA390.1349854631039734No Hit
CCTGTTCGATACCCGCGCTTTNGAGCCTCAGCGTCAGTTGCGCTCCGGTA370.12806313166274402No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG370.12806313166274402No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA360.12460196594212931No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACGGCTTAGTG360.12460196594212931No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTNCGGCA360.12460196594212931No Hit
CCTGTTCGATACCNGCGCTTTCGAGCNTCAGNGTNAGTTACNCTNCCGCC360.12460196594212931No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGNTGCGCTCCGGTA360.12460196594212931No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGNTACGCTCCCGCC360.12460196594212931No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA350.1211408002215146No Hit
CCTGTTNGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNCCGCC350.1211408002215146No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA350.1211408002215146No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA350.1211408002215146No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGCA350.1211408002215146No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA340.1176796345008999No Hit
CCTGTTCGATACCCGCGCTTTNGAGCTTCAGCGTCAGTTACGCTCCCGCC340.1176796345008999No Hit
CCTGTTCGANACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA340.1176796345008999No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA340.1176796345008999No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA330.11421846878028521No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA330.11421846878028521No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA330.11421846878028521No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATCAGCGTCAGTGTGCAACNGGCA330.11421846878028521No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAACGTCAGTTGCAGCTTAGCA320.1107573030596705No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA320.1107573030596705No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTNAGCGTCAGTTACGCTNCCGCC320.1107573030596705No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATCAGCGTNAGTGTGCAACTGGCA320.1107573030596705No Hit
CCTGTTCGATACCNGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA320.1107573030596705No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA320.1107573030596705No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG310.1072961373390558No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA310.1072961373390558No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA310.1072961373390558No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA300.10383497161844109No Hit
CCTGTTTGATACCNACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG300.10383497161844109No Hit
CCTGTTTGATACCCACACTTTNGAGCATGAACGTCAGTTACGGCTTAGTG300.10383497161844109No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTGCAGCTTAGCA300.10383497161844109No Hit
CCTGTTTGANACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG300.10383497161844109No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTNNGGCA300.10383497161844109No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGNGTCAGTTACGCTNNCGCC300.10383497161844109No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTNAGTAATGCTGCGGCA300.10383497161844109No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAACGTCAGTTGCAGCNTAGCA300.10383497161844109No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA300.10383497161844109No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTTAGCA300.10383497161844109No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAACGTCAGTCGCGGCNTAGCA290.10037380589782638No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAACGTCAGTCGCGGCNTAGCA290.10037380589782638No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGNTACGGCTTAGTG290.10037380589782638No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA290.10037380589782638No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTNAGCGTCAGTTGCGCTNCGGTA290.10037380589782638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)