FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21055
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG18408.739016860603183No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG6312.9969128473046784No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG6052.873426739491807No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG5772.740441700308715No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG4422.099263832818808No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG4382.0802659700783663No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA3111.4770838280693421No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGNTGCGCTCCAGTG2851.353597720256471No Hit
CCTGTTCGATACCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG2671.2681073379244834No Hit
CCTGTTCGATACCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCAGTG2541.206364284018048No Hit
CCTGTTCGATACCCGCACTTTNGAGCTTCAGCGTCAGTTGCGCTCCAGTG2271.0781287105200665No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2151.0211351222987415No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTNAGCGTCAGTTGCGCTNCAGTG2151.0211351222987415No Hit
CCTGTTCGANACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG2131.0116361909285205No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG1990.9451436713369745No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1990.9451436713369745No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGNGTCAGTTGCGCTNNAGTG1960.9308952742816434No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1820.8644027546900974No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCNAGTG1490.7076703870814534No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1440.6839230586559012No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG1440.6839230586559012No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNNAGTG1390.6601757302303491No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGTTGCGCCCCAGTG1310.6221800047494657No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1260.5984326763239136No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA1190.5651864165281406No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGAA1140.5414390881025885No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA960.45594870577060087No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGAA940.44644977440038No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGNTGCGCCCCAGTG880.41795298028971745No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG850.40370458323438607No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA830.39420565186416523No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA790.3752077891237236No Hit
CCTGTTCGATCCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG770.36570885775350276No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA760.36095939206839234No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA740.35146046069817144No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG730.346710995013061No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA660.31346473521728807No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGNGTCAGTTGCGCCNNAGTG630.2992163381619568No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA620.2944668724768464No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA620.2944668724768464No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA610.28971740679173597No Hit
CNTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGTTGCGCCCCAGTG580.27546900973640465No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGAA580.27546900973640465No Hit
CCTGTTCGATACCNGCGCTTTCGAGCNTCAGNGTNAGTTGCNCCNCAGTG570.2707195440512942No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA570.2707195440512942No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTNAGCGTCAGTTGCGCCNCAGTG550.2612206126810734No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG540.25647114699596296No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA510.2422227499406317No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA490.23272381857041086No Hit
CCTGTTCGANACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG480.22797435288530044No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA470.22322488720019No Hit
CCTGTTCGATACCNGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG470.22322488720019No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA460.21847542151507957No Hit
CCTGTTNGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG450.21372595582996914No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCNAGCA420.19947755877463783No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA410.19472809308952743No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA410.19472809308952743No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA400.189978627404417No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA390.1852291617193066No Hit
CCTGTTNGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG390.1852291617193066No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCNAGTG390.1852291617193066No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGAA380.18047969603419617No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA380.18047969603419617No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA370.17573023034908572No Hit
CCTGTTCGATACCCGCGCTTTNGAGCTTCAGCGTCAGTTGCGCCCCAGTG370.17573023034908572No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA360.1709807646639753No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA360.1709807646639753No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNNAGCA340.16148183329375446No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA330.15673236760864404No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA330.15673236760864404No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA330.15673236760864404No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA330.15673236760864404No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA310.1472334362384232No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTGTNGTNCAGAA300.14248397055331277No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTGTCGTNCAGAA300.14248397055331277No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTGTCGTNNAGAA300.14248397055331277No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTNNAGTG290.13773450486820232No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA290.13773450486820232No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA280.1329850391830919No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG280.1329850391830919No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA280.1329850391830919No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA280.1329850391830919No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA280.1329850391830919No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA270.12823557349798148No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGCA270.12823557349798148No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTNCAGTG270.12823557349798148No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA270.12823557349798148No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCNNAGTG270.12823557349798148No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA260.12348610781287106No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA260.12348610781287106No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA260.12348610781287106No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA260.12348610781287106No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA260.12348610781287106No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG260.12348610781287106No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA250.11873664212776064No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTGTCGTCCAGAA250.11873664212776064No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA240.11398717644265022No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA240.11398717644265022No Hit
CCTGTTCGATCCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG240.11398717644265022No Hit
CCTGTTTGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG240.11398717644265022No Hit
CCTGTTCGATACCCGCACTTTNGAGCCTCAGCGTCAGTTGCGCTCCAGTG240.11398717644265022No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA240.11398717644265022No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA240.11398717644265022No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTNAGTTGCGCTCCAGTG230.10923771075753978No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA230.10923771075753978No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA220.10448824507242936No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGTA220.10448824507242936No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGNAGCAGTCCAGTA220.10448824507242936No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA220.10448824507242936No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCCCCAGTG220.10448824507242936No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT220.10448824507242936No Hit
ACTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG220.10448824507242936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)