FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002448300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71854
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA1157916.114621315445206No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA998413.89484231914716No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC53067.38441840398586No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA46906.52712444679489No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG45326.307234113619284No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA42295.885545689871127No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG34364.7819188910846995No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA33164.614913574748796No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA22263.0979486180310074No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA20122.800122470565313No Hit
TGAGGAATATTGGTCAATGGGCGATGGCCTGAACCAGCCAAGTAGCGTGA19432.7040944136721685No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA16682.3213738970690567No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA8641.202438277618504No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA6970.9700225457177053No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA5650.7863166977482118No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA5200.7236897041222479No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCAACGCCGCGTGA4740.6596709995268183No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGG4550.6332284911069669No Hit
TAGGGAATCTTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGA4130.5747766303894007No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGA4070.5664263645726055No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA3920.5455507000306176No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC3620.5037993709466418No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA3380.47039830767946117No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA3160.43978066635121216No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2820.3924624933893729No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGT2630.36601998496952154No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGTGCGACGCCGCGTGG2560.3562780081832605No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA2410.3354023436412726No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGA2360.32844378879394326No Hit
TGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCAACGCCGCGTGG2340.3256603668550116No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC2290.3187018120076822No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA2280.3173101010382164No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGATGCCGCGTGG2260.31452667909928467No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1700.23659086480919642No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA1700.23659086480919642No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGATGCCGCGTGA1670.23241573190079884No Hit
TTAGGAATATTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGA1660.231024020931333No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC1480.20597322348094746No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA1420.1976229576641523No Hit
TGAGGAATATTGGTCAATGGGCGTTAGCCTGAACCAGCCAAGTAGCGTGA1210.1683970273053692No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGC1210.1683970273053692No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC1190.1656136053664375No Hit
TGGGGGATATTGGACAATGGGGGAAACCCTGATCCAGCGATGCCGCGTGA1160.1614384724580399No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGA1100.15308820664124476No Hit
TGAGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCCATGCCGCGTGA1070.14891307373284718No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGC1050.14612965179391543No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGATGCCGCGTGA1020.14195451888551786No Hit
TGAGGAATATTGGTCAATGGGCGCTAGCCTGAACCAGCCAAGTAGCGTGA980.1363876750076544No Hit
TGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGA900.12525398725192752No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCAACGCCGCGTGA870.12107885434352994No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCAGCGTGA750.1043783227099396No Hit
TGGGGGATATTGGACAATGGGGGCAACCCTGATCCAGCGACGCCGCGTGA740.10298661174047373No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGG720.10020318980154201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)