FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002448373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81628
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA59467.284265203116577No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA53026.495320233253296No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA49906.113098446611457No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGTGCTCCGGTA26593.2574606752584896No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG25693.147204390650267No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA23312.855637771352967No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA17722.1708237369530066No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA16802.0581173126868233No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA16301.9968638212378105No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA14231.7432743666388983No Hit
CNTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA13381.639143431175577No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA13191.6158671044249524No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA12581.5411378448571569No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA11441.4014798843534082No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA10571.294898809232126No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA10231.2532464350467976No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG7240.8869505561817024No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA6830.836722693193512No Hit
CNTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGTGCTCCGGTA6340.7766942715734797No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA5820.7129906404665066No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA5650.6921644533738424No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG5630.6897143137158818No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA5500.6737884059391386No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA5440.666437986965257No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG5400.661537707649336No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA4980.6100847748321654No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA4220.5169794678296663No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA3930.481452442789239No Hit
CNCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA3560.43612485911696963No Hit
CNTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA3420.4189738815112461No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3230.39569755476062135No Hit
CNCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA3050.37364629783897685No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2920.35772039006223355No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA2750.3368942029695693No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA2480.30381731758710245No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2360.28911647963933945No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2320.28421620032341843No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA2060.2523643847699319No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1950.2388886166511491No Hit
NNTGTTTGNTANCCATGCTTTCGAGCCTCNGCGTCAGTTGGTGCCCAGTA1930.23643847699318862No Hit
NNTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1870.2290880580193071No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1810.2217376390454256No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1800.22051256921644535No Hit
NNTGTTCGNTANCCGCGCTTTCGAGCCTCNGCGTCAGTTGCGCTCCGGTA1790.2192874993874651No Hit
NNTGTTCGATACCNGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA1600.1960111726368403No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1560.1911108933209193No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1540.18866075366295879No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCGTAGCA1430.17518498554417603No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGTCCAGTA1410.17273484588621552No Hit
NNTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1400.17150977605723525No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA1370.16783456657029452No Hit
NNTGTTTGNTANCCACACTTTCGAGCCTCNATGTCAGTTGCAGCTTAGCA1340.16415935708335375No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1280.15680893810947225No Hit
CCTGTTTGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA1270.155583868280492No Hit
CNTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1260.15435879845151174No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1210.14823344930661048No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1200.14700837947763024No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTTCCCAGTA1170.14333316999068949No Hit
CCCGTTTGCTACCCTGGCTTTCGCAGCTCAGCGTCAGACACAGTCCAGAA1150.14088303033272898No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1140.1396579605037487No Hit
CNTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1070.13108247170088697No Hit
CCTGTTCGATACCCGCGCTTTCGCGCCTCAGCGTCAGTTGCGCTCCGGTA1050.12863233204292646No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGTA1000.1225069828980252No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA990.12128191306904493No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCTGGTA990.12128191306904493No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA900.11025628460822268No Hit
CNTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA870.10658107512128191No Hit
NNTGTTCGNTANCCGCGCTTTCGAGCCTCNGCGTCAGTTGTGCTCCGGTA850.10413093546332142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)