FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002448449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211152
Sequences flagged as poor quality0
Sequence length301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT4946323.42530499355914No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG43022.037394862468743No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAG34801.6481018413275743No Hit
CCTGTTTGCTCCCCACGCCTTCGCACCTCAGTGTCAGTATCAGTCCAGGT30261.4330908539819656No Hit
CCTGTTTGCTCCCCACGCTCTCGCACCTCAGTGTCAGTATCAGTCCAGGT28701.3592104266121088No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGGT25241.1953474274456315No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGCT21731.0291164658634537No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCCGGT13650.6464537394862468No Hit
CCTGCTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT10510.49774569978025307No Hit
CCTGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT9310.4409146018034402No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCCGTATCAGTCCAGGT8870.42007653254527544No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCCGTGTCAGTATCAGTCCAGGT7710.36513980450102296No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCCGTCCAGGT7290.34524892020913844No Hit
CCTGTTTGCTACCCATGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG6860.3248844434341138No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCTAGGT6700.3173069637038721No Hit
CCTGTTTGCTCCCCACGCTTTCGCAACTCAGTGTCAGTATCAGTCCAGGT5610.26568538304160033No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGTT5560.26331742062589986No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCACAGTGTCAGTATCAGTCCAGGT5490.2600022732439191No Hit
CCCGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT5330.25242479351367736No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT5320.2519512010305372No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGCT5240.2481624611654164No Hit
CCTGTTTGCTCCACACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT5100.2415321664014549No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG5070.24011138895203457No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT4970.23537546412063345No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGCCCAGGT4070.1927521406380238No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTCTCAGTCCAGGT3910.18517466090778206No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG3910.18517466090778206No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCTGGT3730.17664999621126012No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCCCAGTGTCAGTATCAGTCCAGGT3650.17286125634613927No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACAGACCAGAG3580.16954610896415853No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCATGT3400.1610214442676366No Hit
CCTGTTTGCTCCCCCCGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT3290.1558119269530954No Hit
CCTGTTTGCGCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT3200.15154959460483441No Hit
CCTGTTTGCCCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT2950.13970978252633173No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGCG2890.1368682276274911No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCACTCCAGGT2870.13592104266121088No Hit
CCTGTTTGCTCCCCACGCCCTCGCACCTCAGTGTCAGTATCAGTCCAGGT2690.12739637796468894No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGCATCAGTCCAGGT2480.11745093581874669No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTAACAGTCCAGGT2250.1065583087065242No Hit
CCTGTTTGCTACCCACGCTCTCGAGCCTCAGTGTCAGTTACAGTCCAGAG2230.10561112374024399No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGTT2150.10182238387512314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)