FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52832
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA10511.9893246517262264No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA5841.1053906723198061No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5361.0145366444579043No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA4850.9180042398546336No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA4720.8933979406420353No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA4180.7911871592973956No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA4080.7722592368261659No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA3840.7268322228952151No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA3740.7079043004239854No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA3370.6378709872804361No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA3130.5924439733494852No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT2760.5224106602059359No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCCAGAA2550.4826620230163537No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2460.4656268927922471No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2460.4656268927922471No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA2460.4656268927922471No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA2310.4372350090854028No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2300.43534221683827984No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA2280.43155663234403396No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNNAGGA2220.42019987886129617No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA2100.39748637189582076No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA2050.38802241066020593No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA2030.38423682616596005No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA2010.3804512416717141No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2000.37855844942459116No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1990.3766656571774682No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNNGGTA1900.3596305269533616No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCNAGGA1870.3539521502119927No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1860.35205935796486976No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCCAGAA1780.33691701998788615No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCNGGTA1690.3198818897637795No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1650.3123107207752877No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNNAGAA1640.31041792852816474No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA1630.3085251362810418No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCNAGAA1620.30663234403391887No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA1570.2971683827983041No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1530.28959721380981224No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA1500.28391883706844334No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA1490.28202604482132043No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1460.27634766807995154No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA1390.26309812235009084No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1390.26309812235009084No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA1370.2593125378558449No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCNAGAA1340.25363416111447606No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1310.24795578437310722No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA1310.24795578437310722No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA1310.24795578437310722No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1300.24606299212598426No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1260.23849182313749243No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCACCGTCAGTTGTCGTCCAGCA1260.23849182313749243No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA1260.23849182313749243No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA1250.2365990308903695No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCACNGTNAGTTGTNGTNCAGCA1250.2365990308903695No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1240.2347062386432465No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1230.23281344639612356No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGNTGTCGTCCAGCA1190.22524227740763175No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA1180.22334948516050881No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1150.21767110841913992No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1130.213885523924894No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1120.21199273167777105No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNACCGTCAGTTGTCGTNCAGCA1110.21009993943064809No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTNAGTTAATGTCCAGGA1090.20631435493640216No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTNCAGAA1060.20063597819503332No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1050.19874318594791038No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA1030.19495760145366445No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA1030.19495760145366445No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGCA1030.19495760145366445No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGTT1020.19306480920654148No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCNAGCA1020.19306480920654148No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGTT1000.18927922471229558No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGCA990.18738643246517264No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTNNAGCA980.18549364021804968No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTNNAGAA960.18170805572380377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA960.18170805572380377No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG900.170351302241066No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGTA890.16845850999394307No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA880.1665657177468201No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTNCAGCA880.1665657177468201No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGTT880.1665657177468201No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA850.16088734100545124No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGCA850.16088734100545124No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA840.1589945487583283No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA810.15331617201695943No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGCA800.15142337976983647No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGTA790.1495305875227135No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA790.1495305875227135No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGTA780.14763779527559054No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA780.14763779527559054No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA770.1457450030284676No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNNAGTA770.1457450030284676No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT760.14385221078134464No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGTA760.14385221078134464No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA750.14195941853422167No Hit
CCTGTTTGCTCCCNACACTTTCGTGCNTCAANGTNAGTTACNGTNCAGAA750.14195941853422167No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA740.1400666262870987No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA740.1400666262870987No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGNGTCAGTTAATGTNNAGGA740.1400666262870987No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA730.13817383403997577No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA730.13817383403997577No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG730.13817383403997577No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA730.13817383403997577No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG720.13628104179285283No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA720.13628104179285283No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA720.13628104179285283No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA710.13438824954572987No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA710.13438824954572987No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNCAGAA700.1324954572986069No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCNAGTA690.13060266505148396No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA690.13060266505148396No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA680.128709872804361No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNNAGAA670.12681708055723803No Hit
CCCTTTCGCTCCCNTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA670.12681708055723803No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTNAGCGTCAGTTGCGCTNCGGTA660.12492428831011508No Hit
CCTGTTCGATACCCGCGCTTTNGAGCCTCAGCGTCAGTTGCGCTCCGGTA660.12492428831011508No Hit
CCTGTTCGATACCNGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA650.12303149606299213No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA640.12113870381586916No Hit
CCTGTTCGANACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA630.11924591156874621No Hit
CNTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA620.11735311932162325No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA620.11735311932162325No Hit
CCCTTTNGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA610.1154603270745003No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA600.11356753482737733No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACGGTCCAGAA600.11356753482737733No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA590.11167474258025441No Hit
CCTGTTCGATACCNGCGCTTTCGAGCNTCAGNGTNAGTTGCNCTNCGGTA590.11167474258025441No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA590.11167474258025441No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA590.11167474258025441No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTGTCGTCCAGCA590.11167474258025441No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGNGTCAGTTGCGCTNNGGTA590.11167474258025441No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTAAAAGCCCAGTT590.11167474258025441No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA580.10978195033313144No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA580.10978195033313144No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA580.10978195033313144No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGNTGCGCTCCGGTA580.10978195033313144No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA570.10788915808600849No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA560.10599636583888553No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAANGTCAGTTACTGTNNAGAA560.10599636583888553No Hit
CCCTTTNGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA560.10599636583888553No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTGGTCCAGTA550.10410357359176257No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGTA550.10410357359176257No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCNAGAA550.10410357359176257No Hit
CCTGTTTGCTACCNACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA550.10410357359176257No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA550.10410357359176257No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA550.10410357359176257No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA550.10410357359176257No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA540.10221078134463961No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG540.10221078134463961No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA540.10221078134463961No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA540.10221078134463961No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTGTCGTCCAGCA530.10031798909751666No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA530.10031798909751666No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA530.10031798909751666No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA530.10031798909751666No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA530.10031798909751666No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA530.10031798909751666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)