FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45236
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG501511.086302944557431No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA455210.062781855159606No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA36198.000265275444336No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTAGCGTGA33567.418869926607127No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA26065.7608983995048195No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA21144.673269077725705No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA20784.593686444424795No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA18384.063135555752056No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA14023.099301441329914No Hit
TGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGA11952.641701299849677No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA11552.5532761517375544No Hit
TGGGGGATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA11232.4825360332478557No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGA10122.237156247236714No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA10102.232734989831108No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGATGCCGCGTGA9212.035989035281634No Hit
TGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCAACGCCGCGTGG7811.7265010168892032No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC6091.346272880007074No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC5641.2467945883809355No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGG5501.2158457865416925No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA5081.1229993810239634No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGG5011.1075249801043416No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGTGCGACGCCGCGTGG3940.8709877089044125No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA3840.8488814218763817No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA3460.7648775311698647No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2630.5813953488372093No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG2540.5614996905119817No Hit
TAGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA2510.5548678044035723No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA2460.543814660889557No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA2370.5239190025643293No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTCGCGTGA2270.5018127155362986No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT2100.46423202758864623No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGA2000.44212574056061543No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTCGCGTGG1560.34485807763728005No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA1480.3271730480148554No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGA1400.3094880183924308No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC1240.2741179591475816No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCTATTCCGCGTGT1180.26085418693076307No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1100.24316915730833846No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGATGCCGCGTGG1090.2409585286055354No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA1070.23653727119992926No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA1050.23211601379432312No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCGACGCCGCGTGA940.20779909806348928No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGG920.20337784065788309No Hit
TGAGGAATATTGGTCAATGGACGGGAGTCTGAACCAGCCAAGTAGCGTGA880.1945353258466708No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGC850.18790343973826157No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCAACGCCGCGTGA820.18127155362985234No Hit
TGGGGGATATTGCACAATGGAGGGAACTCTGATGCAGCGACGCCGCGTGA710.15695463789901848No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA610.1348483508709877No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGC610.1348483508709877No Hit
TTGGGAATATTGGACAATGGAGGAAACTCTGATCCAGTGACGCCGCGTGA610.1348483508709877No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA590.13042709346538153No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTAGCGTGA580.12821646476257847No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGGGCGACGCCGCGTGG560.12379520735697233No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA550.12158457865416923No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA530.11716332124856309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)