FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38295
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGGACAATGGGCGGAAGCCTGATCCAGCCATGCCGCGTGA604915.795795795795794No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTAGCGTGG28757.5075075075075075No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA22335.831048439744092No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA18444.8152500326413366No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTAGCGTGC15073.9352395874135007No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA11142.9089959524742133No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG11092.895939417678548No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA10042.6217521869695783No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA8632.2535579057318187No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA8312.1699960830395613No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGT8292.1647734691212954No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG8182.1360490925708318No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGTGCGACGCCGCGTGG7621.989815902859381No Hit
TGGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCAATGCCGCGTGA7431.9402010706358532No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTAGCGTGA6861.7913565739652697No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA6391.6686251468860165No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA6201.6190103146624886No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGC6041.5772294033163599No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA5871.532837185011098No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT5181.3526570048309179No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC4951.2925969447708578No Hit
TGAGGAATATTGGTCAATGGGCGATAGCCTGAACCAGCCAAGTAGCGTGG4491.1724768246507378No Hit
TGGGGAATCTTGCGCAATGGGGGGAACCCTGACGCAGCGACGCCGCGTGC4411.1515863689776733No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA4391.1463637550594072No Hit
TGAGGAATATTGGTCAATGGCCGGGAGGCTGAACCAGCCAAGTAGCGTGA4311.125473299386343No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA4171.0889150019584803No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGA4011.0471340906123516No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA2920.7625016320668494No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGATGCCGCGTGA2650.6919963441702572No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA2500.6528267397832614No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA2260.5901553727640684No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA2250.5875440658049353No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTCGCGTGA2050.5353179266222745No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA1810.47264655960308133No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA1800.4700352526439483No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAACGCCGCGTGA1710.44653349001175086No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1700.4439221830526178No Hit
TGGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCAACGCCGCGTGA1560.40736388562475523No Hit
TGAGGGATATTGGTCAATGGGGGAAACCCTGAACCAGCAACGCCGCGTGA1530.3995299647473561No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA1470.3838621229925578No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1290.33685859772816296No Hit
TGGGGGATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1260.3290246768507638No Hit
TGAGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCATGCCGCGTGC1220.31857944901423163No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGC1160.30291160725943334No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA1070.27940984462723595No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGC1040.2715759237498368No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGA1000.2611306959133046No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC890.2324063193628411No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC840.21934978456717588No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGG830.21673847760804282No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA830.21673847760804282No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTCGCGTGA810.21151586368977673No Hit
TGGGGGATATTGCGCAATGGGGGAAACCCTGACGCAGCAATGCCGCGTGA710.18540279409844626No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGA650.169734952343648No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA640.16712364538451496No Hit
TGAGGAATATTGGACAATGGGCGGAAGCCTGATCCAGCCATGCAGCGTGA640.16712364538451496No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA630.1645123384253819No Hit
TGGGGGATATTGGACAATGGGGGCAACCCTGATCCAGCGACGCCGCGTGA590.15406711058884973No Hit
TGAGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCCATGCCGCGTGA550.14362188275231755No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA550.14362188275231755No Hit
TGAGGGATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGA500.1305653479566523No Hit
TGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA480.1253427340383862No Hit
TGAGGAATATTGGACAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGA470.12273142707925316No Hit
TGGGGAATATTGGACAATGGGCGGAAGCCTGATCCAGCCATGCCGCGTGA450.11750881316098707No Hit
TGGGGGATATTGGTCAATGGGGGAAACCCTGAACCAGCAATGCCGCGTGA430.11228619924272099No Hit
TGGGGAATATTGGGCAATGGGGGCAACCCTGACCCAGCAACGCCGCGTGA420.10967489228358794No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTTACCCAGCAACGCCGCGTGA420.10967489228358794No Hit
TGGGGAATATTGCGCAATGGGGGGAACCCTGACGCAGCAACGCCGCGTGA410.1070635853244549No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGATGCCGCGTGG390.10184097140618881No Hit
TGAGGAATATTGGTCAATGGACGTAAGTCTGAACCAGCCAAGTCGCGTGA390.10184097140618881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)