FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002448709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56596
Sequences flagged as poor quality0
Sequence length301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA32625.763658209060711No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA19193.390698989327868No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT13272.3446886705774257No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA13272.3446886705774257No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA11722.0708177256343205No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA10501.8552547883242632No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA7941.4029260018375855No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7201.2721747119937805No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA6621.1696939713053927No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA5751.0159728602728109No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA5110.9028906636511415No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA5060.8940561170400735No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG4660.8233797441515301No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA4480.7915753763516856No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA4290.7580040992296275No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG3710.6555233585412397No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3320.5866138949749099No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA3210.5671778924305605No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2460.4346596932645417No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2290.40462223478691073No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1880.3321789525761538No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1760.3109760407095908No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1750.3092091313873772No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1720.30390840342073644No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1330.2349989398544067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1250.220863665276698No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1250.220863665276698No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA1160.20496148137677575No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1110.19612693476570783No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1100.19436002544349426No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA1080.1908262067990671No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1070.1890592974768535No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA970.17139020425471765No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT940.1660894762880769No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA920.16255565764364974No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA900.15902183899922256No Hit
CCTGTTTGCTACCCACACCTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA860.15195420171036822No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAGCGTCAGTTATCGTCCAGTA840.14842038306594105No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGCTGCCCAGTA820.14488656442151387No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCCGTTGGTGCCCAGTA780.13781892713265956No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA740.1307512898438052No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGCA730.1289843805215916No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA730.1289843805215916No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGCA720.12721747119937804No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGCA710.12545056187716447No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG700.12368365255495087No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGCG680.12014983391052372No Hit
CCTGCTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA660.11661601526609655No Hit
CCTGTTTGATACCCACACTCTCGAGCATCAGCGTCAGTTACAGTCCAGCA640.11308219662166938No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA610.10778146865502863No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG590.10424765001060146No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA580.10248074068838788No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCCGCGTCAGTTGGTGCCCAGTA580.10248074068838788No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT580.10248074068838788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)