FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15481
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA227014.663135456365866No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA13028.410309411536723No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA11327.312189135068794No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA10466.756669465796783No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA10176.569343065693431No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA7815.044893740714424No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA6834.411859698985854No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA6494.192235643692268No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA5823.7594470641431434No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA5303.423551450164718No Hit
TGGGGGATATTGGACAATGGGGGCAACCCTGATCCAGCGACGCCGCGTGA4502.906788967120987No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA3592.318971642658743No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG3502.260835863316323No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC2551.6471804147018927No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCAAGTAGCGTGA2511.6213422905497061No Hit
TGGGGAATCTTGCGCAATGGGGGGAACCCTGACGCAGCGACGCCGCGTGC2071.337122924875654No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA2041.317744331761514No Hit
TGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGA1821.1756346489244882No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA1791.156256055810348No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG1771.143336993734255No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGA1530.9883082488211357No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC1100.7105484141851302No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA1040.6717912279568503No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGC920.5942768555002906No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA860.5555196692720109No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC860.5555196692720109No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA750.4844648278534978No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGC700.4521671726632646No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGT680.43924811058717134No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCTATTCCGCGTGT660.4263290485110781No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCAACGCCGCGTGA640.41340998643498483No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA630.40695045539693814No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT560.3617337381306117No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGA510.3294360829403785No Hit
TGAGGAATATTGGTCAATGGGCGAAAGCCTGAACCAGCCAAGTAGCGTGA450.2906788967120987No Hit
TGGGGAATATTGCACAATGGAGGCAACTCTGATGCAGCGACGCCGCGTGA450.2906788967120987No Hit
TGAGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCATGCCGCGTGC400.2583812415218655No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA400.2583812415218655No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA380.24546217944577226No Hit
GGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA310.20024546217944578No Hit
TCGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA310.20024546217944578No Hit
TGGGGGATATTGCACAATGGGGGGAACCCTGATGCAGCAACGCCGCGTGA300.19378593114139914No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAATGCCGCGTGA300.19378593114139914No Hit
TAGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCAACACCGCGTGA260.16794780698921258No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA260.16794780698921258No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA250.16148827595116594No Hit
TGGGGAATATTGGGCAATGGGGGCAACCCTGACCCAGCAACGCCGCGTGA250.16148827595116594No Hit
TGGGGAATATTGGTCAATGGGGGGAACCCTGAACCAGCGACGCCGCGTGA230.14856921387507266No Hit
TGGGGGATATTGCACAATGGAGGGAACTCTGATGCAGCAACGCCGCGTGA220.14210968283702605No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGA210.1356501517989794No Hit
TGGGGAATTTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGC200.12919062076093274No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA190.12273108972288613No Hit
TGGGGGATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGA180.11627155868483949No Hit
TGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA170.10981202764679283No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTCGCGTGA160.10335249660874621No Hit
TAGGGAATATTGGGCAATGGGGGCAACCCTGACCCAGCAACGCCGCGTGA160.10335249660874621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)