FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15481
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA5773.72714940895291No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2411.5567469801692397No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2031.3112848007234674No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA1871.2079323041147214No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA1851.195013242038628No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1811.1691751178864416No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1691.0916607454298817No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC1601.033524966087462No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1591.0270654350494153No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1530.9883082488211357No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1170.7557651314514566No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA1170.7557651314514566No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA1150.7428460693753633No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1000.6459531038046638No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA930.6007363865383374No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA830.536141076157871No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA770.4973838899295911No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCNCAGAC700.4521671726632646No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA700.4521671726632646No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNNAGCA690.44570764162521803No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA680.43924811058717134No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA680.43924811058717134No Hit
CCTGTTTGCNACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA680.43924811058717134No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA670.43278857954912475No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA660.4263290485110781No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA660.4263290485110781No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA660.4263290485110781No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCNNAGAC640.41340998643498483No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA620.40049092435889155No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA610.3940313933208449No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA590.38111233124475163No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGNTAAAGCCCAGCA590.38111233124475163No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGNGTCAGTTAAAGCNNAGCA570.36819326916865835No Hit
CCTGTTTGCTACCNACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA570.36819326916865835No Hit
CCTGTTTGCTACCNACGCTTTCGTGCNTCAGNGTNAGTTAANGCNCAGCA560.3617337381306117No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA560.3617337381306117No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA560.3617337381306117No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCNAGTG550.3552742070925651No Hit
CCTGTTTGCTACCCACGCTTTNGTGCCTCAGCGTCAGTTAAAGCCCAGCA550.3552742070925651No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA540.34881467605451844No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCNAGCA530.3423551450164718No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGCA520.33589561397842516No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA510.3294360829403785No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGCA510.3294360829403785No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTNAGCGTCAGTTAAAGCNCAGCA510.3294360829403785No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA500.3229765519023319No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA490.3165170208642853No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG490.3165170208642853No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCNAGAC480.3100574898262386No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTNAGTTGCGGCCCAGAC470.30359795878819196No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTNNAGTG470.30359795878819196No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA440.2842193656740521No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA440.2842193656740521No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA430.27775983463600545No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA430.27775983463600545No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA430.27775983463600545No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGCA420.2713003035979588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA420.2713003035979588No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTNCAGTG420.2713003035979588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGCA410.2648407725599121No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG380.24546217944577226No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA380.24546217944577226No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA380.24546217944577226No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA360.23254311736967898No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA360.23254311736967898No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA360.23254311736967898No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA360.23254311736967898No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA360.23254311736967898No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA360.23254311736967898No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGCA340.21962405529358567No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA340.21962405529358567No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTAAAAGCCCAGCA340.21962405529358567No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA320.20670499321749242No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA320.20670499321749242No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACACTCCAGTG320.20670499321749242No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA320.20670499321749242No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA310.20024546217944578No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAGTCCAGCA310.20024546217944578No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA310.20024546217944578No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG310.20024546217944578No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA310.20024546217944578No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA300.19378593114139914No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA300.19378593114139914No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTNAGNTGCGGCCCAGAC300.19378593114139914No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACACTNNAGTG290.1873264001033525No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA280.18086686906530586No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA280.18086686906530586No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA280.18086686906530586No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA280.18086686906530586No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA280.18086686906530586No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATNAGCGTCAGTTGCGGCNCAGAC270.17440733802725922No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAGTNNAGCA270.17440733802725922No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGNGTCAGTTGCGGCNNAGAC260.16794780698921258No Hit
CCTATTTGCTCCCNACGCTTTCGGGAATGAGCGTCAGTTGCGGCNCAGAC260.16794780698921258No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA260.16794780698921258No Hit
CCTATTNGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC260.16794780698921258No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTNTAGTG260.16794780698921258No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA260.16794780698921258No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA260.16794780698921258No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA250.16148827595116594No Hit
CCTATTTGCTCCCCACGCTTTNGGGAATGAGCGTCAGTTGCGGCCCAGAC250.16148827595116594No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA250.16148827595116594No Hit
CCTATTNGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCNCAGAC240.1550287449131193No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACACTCCAGTG240.1550287449131193No Hit
CCTATTTGCNCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCNCAGAC240.1550287449131193No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTTGCGCTCCGGAC230.14856921387507266No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA230.14856921387507266No Hit
CCTGTTTGCTCCCNACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA230.14856921387507266No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT230.14856921387507266No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA220.14210968283702605No Hit
CNTATTTGCTCCCCACGCTTTCGGGAATGAGCGTNAGTTGCGGCCCAGAC210.1356501517989794No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA210.1356501517989794No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATAGCTTGGAA210.1356501517989794No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA210.1356501517989794No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA200.12919062076093274No Hit
CCTATTTGCTCCCNACGCTTTCGGGANTGAGNGTNAGTTGCNGCNCAGAC200.12919062076093274No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTNAGCGTCAGTTAAAGCNCAGCA200.12919062076093274No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA200.12919062076093274No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA200.12919062076093274No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA200.12919062076093274No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG200.12919062076093274No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACACTCCAGTG200.12919062076093274No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA200.12919062076093274No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAAAAGCCCAGCA200.12919062076093274No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA190.12273108972288613No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA190.12273108972288613No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA190.12273108972288613No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA190.12273108972288613No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACACTNCAGTG190.12273108972288613No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG190.12273108972288613No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAGAGCCCAGCA190.12273108972288613No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA190.12273108972288613No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA190.12273108972288613No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA180.11627155868483949No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNACCGTCAGTTGTCGTNCAGCA180.11627155868483949No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA180.11627155868483949No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNNGGTA180.11627155868483949No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA180.11627155868483949No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA170.10981202764679283No Hit
CCTGTTTGCTCCCNACACTTTCGAGCNTCAGNGTNAGTTAGNGCNCAGCA170.10981202764679283No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCACCGTCAGTTGTCGTCCAGCA170.10981202764679283No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA170.10981202764679283No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACACTNCAGTG170.10981202764679283No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGNGTCAGTTAGAGCNNAGCA170.10981202764679283No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACACTCCAGTG170.10981202764679283No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA170.10981202764679283No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCNGGTA170.10981202764679283No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA170.10981202764679283No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA160.10335249660874621No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA160.10335249660874621No Hit
CCTGTTTGCTCCCNACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA160.10335249660874621No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA160.10335249660874621No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTNNAGCA160.10335249660874621No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA160.10335249660874621No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA160.10335249660874621No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAAAGCCCAGCA160.10335249660874621No Hit
CCTGTTTGCNCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA160.10335249660874621No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA160.10335249660874621No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTNAGCGTCAGTTAGAGCNCAGCA160.10335249660874621No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCNAGTG160.10335249660874621No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTNCAGCA160.10335249660874621No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCNNAGAG160.10335249660874621No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA160.10335249660874621No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTTTCGTCCAGAA160.10335249660874621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)