FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17990
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4792.6625903279599776No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3952.195664257921067No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA2691.4952751528627015No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2391.3285158421345191No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2341.3007226236798222No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT2231.2395775430794886No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA1770.9838799332962757No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1590.8838243468593663No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA1580.8782657031684269No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1540.8560311284046693No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1430.7948860478043357No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA1400.7782101167315175No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA1360.7559755419677598No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1350.7504168982768205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA1180.6559199555308505No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1160.6448026681489716No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1090.6058921623123958No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA1080.6003335186214565No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1060.5892162312395776No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1040.5780989438576988No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA1030.5725403001667593No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT970.5391884380211228No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA950.528071150639244No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA910.5058365758754864No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA850.4724847137298499No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA820.45580878265703173No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA820.45580878265703173No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA810.45025013896609223No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT800.44469149527515284No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA800.44469149527515284No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA800.44469149527515284No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA730.40578098943857704No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA720.40022234574763754No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT680.3779877709838799No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA660.3668704836020011No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA660.3668704836020011No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA650.3613118399110617No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA630.3501945525291829No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACAGTNCAGTA610.3390772651473041No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGTTACAGTCCAGTT610.3390772651473041No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA600.33351862145636463No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA590.32795997776542524No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA570.3168426903835464No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA560.311284046692607No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA560.311284046692607No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACAGTCCAGTA550.3057254030016676No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA540.3001667593107282No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA530.2946081156197888No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACAGTCCAGTA530.2946081156197888No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA520.2890494719288494No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA520.2890494719288494No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA510.28349082823790994No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA510.28349082823790994No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA500.27793218454697055No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA490.2723735408560311No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA490.2723735408560311No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA480.2668148971650917No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA480.2668148971650917No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACAGTNNAGTA470.26125625347415227No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA470.26125625347415227No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA460.2556976097832129No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNNAGAA460.2556976097832129No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA460.2556976097832129No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGCA460.2556976097832129No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA450.2501389660922735No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG450.2501389660922735No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGNGTCAGTAGCAGTNNAGTA430.23902167871039467No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA420.23346303501945526No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGAGCCGGCA420.23346303501945526No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA410.22790439132851587No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA400.22234574763757642No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA400.22234574763757642No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA380.2112284602556976No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCNAGAA380.2112284602556976No Hit
CCTGTTTGCNCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA360.20011117287381877No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGNTACAGTCCAGTT360.20011117287381877No Hit
CCTGTTTGCNCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT350.19455252918287938No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA340.18899388549193996No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA340.18899388549193996No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA340.18899388549193996No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGNAGCAGTCCAGTA330.18343524180100054No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCNTCAGNGTNAGTAGCNGTNCAGTA330.18343524180100054No Hit
CCTGTTTGCTCCCCACGCTTTNGTGCCTCAGTGTCAGTAGCAGTCCAGTA330.18343524180100054No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA330.18343524180100054No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA330.18343524180100054No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA320.17787659811006115No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA320.17787659811006115No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA310.17231795441912173No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA310.17231795441912173No Hit
CCTGTTTGCTCCCCACACTTTNGCGCCTCAGCGTCAGTTACAGTCCAGTT310.17231795441912173No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTNAGTGTCAGTAGCAGTNCAGTA310.17231795441912173No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA300.16675931072818231No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA300.16675931072818231No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGCA300.16675931072818231No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA300.16675931072818231No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA300.16675931072818231No Hit
CNTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGTTACAGTCCAGTT300.16675931072818231No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA290.1612006670372429No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA290.1612006670372429No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA290.1612006670372429No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGNGTCAGTTACAGTNNAGTT280.1556420233463035No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA280.1556420233463035No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA280.1556420233463035No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA280.1556420233463035No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA280.1556420233463035No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA280.1556420233463035No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA270.1500833796553641No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCGGTATCGGTCTGGCG270.1500833796553641No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA270.1500833796553641No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA270.1500833796553641No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA270.1500833796553641No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA260.1445247359644247No Hit
CCTGTTTGCTCCCNACACTTTCGCGCNTCAGNGTNAGTTACNGTNCAGTT260.1445247359644247No Hit
CCTGTTNGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT260.1445247359644247No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA260.1445247359644247No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA260.1445247359644247No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA260.1445247359644247No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA260.1445247359644247No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA250.13896609227348528No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTNAGCGTCAGTTACAGTNCAGTT250.13896609227348528No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA250.13896609227348528No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA250.13896609227348528No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA250.13896609227348528No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA250.13896609227348528No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC250.13896609227348528No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA240.13340744858254586No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA240.13340744858254586No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA240.13340744858254586No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA240.13340744858254586No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA240.13340744858254586No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA230.12784880489160644No Hit
CCTGTTTGCTCCCNACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT230.12784880489160644No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA230.12784880489160644No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA230.12784880489160644No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA220.12229016120066703No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA220.12229016120066703No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA220.12229016120066703No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA220.12229016120066703No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNCAGCA210.11673151750972763No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA210.11673151750972763No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA210.11673151750972763No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTNAACGTCAGATATAGTNTGGTA210.11673151750972763No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT210.11673151750972763No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA210.11673151750972763No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA210.11673151750972763No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA200.11117287381878821No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNNAGCA200.11117287381878821No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA200.11117287381878821No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA200.11117287381878821No Hit
CCTGTTNGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT200.11117287381878821No Hit
CCTGTTCGATACCCACGCCTTCGTGCTTCAGCGTCAGTTGAGCGCCGGTA200.11117287381878821No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA190.1056142301278488No Hit
CNTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA190.1056142301278488No Hit
CCTGTTTGCTCCCNACACTTTCGTGCNTCAANGTNAGTTACNGTNCAGAA190.1056142301278488No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGANCAGAC180.10005558643690939No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTNAACGTCAGTTACTGTNCAGAA180.10005558643690939No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGTG180.10005558643690939No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGAGNNGGCA180.10005558643690939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)