FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002448805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002448805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61750
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG16302.639676113360324No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA13492.1846153846153844No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA12382.0048582995951416No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA9731.5757085020242916No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG6351.0283400809716599No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA6341.0267206477732793No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG5630.911740890688259No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA5460.8842105263157894No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG5450.8825910931174088No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG5340.8647773279352227No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA5300.8582995951417004No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA4800.7773279352226721No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA4520.7319838056680161No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA4340.702834008097166No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA4310.6979757085020243No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA3990.6461538461538461No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG3940.6380566801619433No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA3690.5975708502024292No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA3640.5894736842105264No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3600.5829959514170041No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA3300.534412955465587No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3150.5101214574898786No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA2750.44534412955465585No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA2710.4388663967611336No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGAA2580.4178137651821862No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2560.4145748987854251No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA2560.4145748987854251No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGTG2360.3821862348178137No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA2320.37570850202429146No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2180.35303643724696354No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGTG2120.34331983805668015No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGAA2040.3303643724696356No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG1950.3157894736842105No Hit
CCTGTTCGATACCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1950.3157894736842105No Hit
CCTGTTCGATACCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCAGTG1950.3157894736842105No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1900.3076923076923077No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1890.3060728744939271No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1890.3060728744939271No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA1890.3060728744939271No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1850.29959514170040485No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGNGTCAGTTGCGCTNNAGTG1840.2979757085020243No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGNTGCGCTCCAGTG1840.2979757085020243No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG1830.2963562753036437No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1820.2947368421052632No Hit
CCTGTTCGATACCCGCACTTTNGAGCTTCAGCGTCAGTTGCGCTCCAGTG1810.2931174089068826No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA1800.29149797570850206No Hit
CCTGTTCGANACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1800.29149797570850206No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1790.28987854251012146No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1770.28663967611336033No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1740.28178137651821866No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1710.2769230769230769No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA1700.27530364372469635No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1690.27368421052631575No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1670.2704453441295546No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTNAGCGTCAGTTGCGCTNCAGTG1670.2704453441295546No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1640.26558704453441295No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1630.26396761133603236No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1590.2574898785425101No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1590.2574898785425101No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACAGTNNAGTA1570.25425101214574897No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1560.25263157894736843No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1540.2493927125506073No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1530.24777327935222673No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1530.24777327935222673No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1530.24777327935222673No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1520.24615384615384617No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1450.2348178137651822No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1440.23319838056680164No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1430.23157894736842108No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTNAGCA1410.2283400809716599No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACAGTCCAGTA1410.2283400809716599No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNTAGCA1400.22672064777327933No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA1370.22186234817813763No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGTG1360.22024291497975707No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACAGTCCAGTA1350.2186234817813765No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1340.21700404858299593No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1330.21538461538461537No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1330.21538461538461537No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGAA1330.21538461538461537No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA1320.2137651821862348No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA1320.2137651821862348No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1320.2137651821862348No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNNAGCA1280.20728744939271254No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACAGTNCAGTA1260.2040485829959514No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA1170.1894736842105263No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGNGTCAGTAGCAGTNNAGTA1170.1894736842105263No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA1150.18623481781376516No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1130.18299595141700403No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA1130.18299595141700403No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGNAGCAGTCCAGTA1100.17813765182186234No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCNTCAGNGTNAGTAGCNGTNCAGTA1070.17327935222672064No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1070.17327935222672064No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA1050.1700404858299595No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTNAGTA1040.16842105263157894No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1030.16680161943319838No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTNAGTGTCAGTAGCAGTNCAGTA990.16032388663967612No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGCA990.16032388663967612No Hit
CCTGTTTGCNCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA980.15870445344129555No Hit
CCTGTTTGCTCCCCACGCTTTNGTGCCTCAGTGTCAGTAGCAGTCCAGTA980.15870445344129555No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA970.15708502024291499No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNNAGTA950.15384615384615385No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG910.1473684210526316No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA870.14089068825910933No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGTA870.14089068825910933No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA860.13927125506072874No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGTA860.13927125506072874No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA860.13927125506072874No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNNAGCA850.13765182186234817No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTNAGTTACAGCTTAGCA840.1360323886639676No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA840.1360323886639676No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG820.13279352226720648No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA820.13279352226720648No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGAA810.1311740890688259No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA810.1311740890688259No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA810.1311740890688259No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNTAGTA790.12793522267206478No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTGTCGTCCAGAA790.12793522267206478No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA790.12793522267206478No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGCA780.12631578947368421No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTACNGTNCAGTG780.12631578947368421No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA770.12469635627530365No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA760.12307692307692308No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA750.12145748987854252No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG750.12145748987854252No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCNAGGT750.12145748987854252No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA740.11983805668016195No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG740.11983805668016195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTGTCGTNNAGAA740.11983805668016195No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA740.11983805668016195No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTNNAGGT740.11983805668016195No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTNCAGGT730.11821862348178139No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTGTCGTNCAGAA720.11659919028340082No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGTG720.11659919028340082No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG720.11659919028340082No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT720.11659919028340082No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGTA720.11659919028340082No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCNAGCA710.11497975708502023No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTA710.11497975708502023No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTACAGTNNAGTG710.11497975708502023No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG680.11012145748987853No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA680.11012145748987853No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGNTACAGTCCAGTG680.11012145748987853No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNCAGCA680.11012145748987853No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGTGTCAGTTACAGTNCAGTG660.1068825910931174No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTA660.1068825910931174No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGTGTCAGTTACAGTCCAGTG650.10526315789473684No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA650.10526315789473684No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA650.10526315789473684No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTGTNGTNCAGAA650.10526315789473684No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA640.10364372469635627No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCNAGAA630.1020242914979757No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTNAGTTACAGGTTAGTA630.1020242914979757No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA620.10040485829959514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)