FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20226
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4472.210026698309107No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA4202.076535152773658No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4011.9825966577672303No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2851.4090774250964107No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA2561.2656976169287057No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA2131.053099970335212No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA1950.9641056066449124No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA1910.9443290813804014No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA1800.8899436369029962No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1690.8355581924255908No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1670.8256699297933353No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA1610.7960051418965688No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1600.7910610105804411No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1570.7762286166320578No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1480.7317314347869079No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1340.6625135963611193No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA1250.6180164145159696No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1210.5982398892514585No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1150.568575101354692No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA1110.5487985760901809No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA1080.5339661821417977No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1080.5339661821417977No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1030.5092455255611589No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1020.5043013942450312No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA930.4598042123998813No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA910.4499159497676259No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA900.4449718184514981No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTNAGCA860.425195293186987No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNTAGCA860.425195293186987No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA850.4202511618708593No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNNAGCA840.4153070305547315No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNNAGCA840.4153070305547315No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTNAGCA790.3905863739740928No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA790.3905863739740928No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA780.385642242657965No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA730.3609215860773262No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGTG720.35597745476119846No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA710.3510333234450707No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA710.3510333234450707No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA710.3510333234450707No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA690.3411450608128152No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA680.33620092949668745No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA670.33125679818055964No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG670.33125679818055964No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA670.33125679818055964No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACAGTNNAGTA650.32136853554830414No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACAGTNCAGTA640.31642440423217644No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA630.3114802729160487No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA590.2917037476515376No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA590.2917037476515376No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA590.2917037476515376No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA580.28675961633540986No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGTG580.28675961633540986No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA570.2818154850192821No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA550.2719272223870266No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA540.26698309107089885No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA540.26698309107089885No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA540.26698309107089885No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA530.2620389597547711No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA530.2620389597547711No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA520.25709482843864334No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA520.25709482843864334No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA520.25709482843864334No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTNAGTGGCGGCTTAGCA510.2521506971225156No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACAGTCCAGTA510.2521506971225156No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA510.2521506971225156No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACAGTCCAGTA510.2521506971225156No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA500.2472065658063878No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNNAGTG500.2472065658063878No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA490.24226243449026008No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA480.23731830317413233No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA480.23731830317413233No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA480.23731830317413233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA470.23237417185800455No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA460.2274300405418768No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA460.2274300405418768No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCTAGAA460.2274300405418768No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA450.22248590922574904No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA440.21754177790962126No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCNGGCA430.2125976465934935No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCNNGGCA430.2125976465934935No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA430.2125976465934935No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA430.2125976465934935No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA420.20765351527736575No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA420.20765351527736575No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA420.20765351527736575No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCNAGAA420.20765351527736575No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA420.20765351527736575No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA410.20270938396123803No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA410.20270938396123803No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCNAGAA410.20270938396123803No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG410.20270938396123803No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA410.20270938396123803No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNNAGAA390.1928211213289825No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA390.1928211213289825No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA380.18787699001285474No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTNCAGAA380.18787699001285474No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA370.18293285869672699No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA370.18293285869672699No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTNNAGAA370.18293285869672699No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCNCGGCA370.18293285869672699No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACGGCTTAGTG360.17798872738059923No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG340.16810046474834373No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA340.16810046474834373No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA340.16810046474834373No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA330.16315633343221594No Hit
CCTGTTTGATACCCGCACTTTNGAGCCTCAACGTCAGTGGCGGCTTAGCA330.16315633343221594No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTNAGTG330.16315633343221594No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA330.16315633343221594No Hit
CCTGTTTGATACCNACACTTTCGAGCNTGAANGTNAGTTACNGTNTAGCA330.16315633343221594No Hit
CNTGTTTGATACCCGCACTTTCGAGCCTCAACGTNAGTGGCGGCTTAGCA320.15821220211608822No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA320.15821220211608822No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA320.15821220211608822No Hit
CCTGTTNGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNTAGCA320.15821220211608822No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGCA310.15326807079996044No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA310.15326807079996044No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA310.15326807079996044No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA300.14832393948383268No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA300.14832393948383268No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA300.14832393948383268No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA290.14337980816770493No Hit
CCTGTTTGATACCCACACTTTNGAGCATGAACGTCAGTTACAGTTTAGCA290.14337980816770493No Hit
CCTGTTTGATACCNGCACTTTCGAGCCTCAACGTCAGTGGCGGCNTAGCA290.14337980816770493No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA280.13843567685157718No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGNTACAGTTTAGCA280.13843567685157718No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTNAGTGCTGCCCCGGCA280.13843567685157718No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA280.13843567685157718No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGTG280.13843567685157718No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA280.13843567685157718No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA270.13349154553544942No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA270.13349154553544942No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA270.13349154553544942No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA270.13349154553544942No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA260.12854741421932167No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA260.12854741421932167No Hit
CCTGTTNGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA260.12854741421932167No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGCCCAGTA250.1236032829031939No Hit
CCTGTTTGATACCNACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA240.11865915158706616No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA240.11865915158706616No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA240.11865915158706616No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCNAGAA230.1137150202709384No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA230.1137150202709384No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTACNGTNCAGTG230.1137150202709384No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA230.1137150202709384No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTACAGTNNAGTG230.1137150202709384No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA230.1137150202709384No Hit
CCTGTTTGATACCNGCACTTTCGAGCNTCAANGTNAGTGGCNGCNTAGCA230.1137150202709384No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA230.1137150202709384No Hit
CCTGTTTGANACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNTAGCA220.10877088895481063No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNCAGCA220.10877088895481063No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTNAGNGGCGGCTTAGCA220.10877088895481063No Hit
CCTGTTTGANACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA220.10877088895481063No Hit
CCTGTTTGCTCCCCACGCTTTNGTGCCTCAGTGTCAGTAGCAGTCCAGTA220.10877088895481063No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGTGTCAGTTACAGTNCAGTG220.10877088895481063No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG220.10877088895481063No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGNGTCAGTAGCAGTNNAGTA220.10877088895481063No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG210.10382675763868288No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA210.10382675763868288No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNNAGCA210.10382675763868288No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA210.10382675763868288No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCGGTA210.10382675763868288No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAACGTCAGTTACAGTNTAGCA210.10382675763868288No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA210.10382675763868288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)