FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15891
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA8655.443332704046315No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG3642.2906047448241145No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA3542.2276760430432323No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA3232.032597067522497No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2961.862689572714115No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2861.7997608709332327No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA2701.699074948083821No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2231.4033100497136746No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2171.365552828645145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2121.334088477754704No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1410.8872946951104398No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1240.78031590208294No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCCCGCC1210.7614372915486753No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA1200.7551444213705871No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1200.7551444213705871No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG1190.7488515511924989No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1140.7173872003020577No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA1130.7110943301239695No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1120.7048014599458813No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG1070.6733371090554402No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1050.6607513686992637No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1020.641872758164999No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1000.6292870178088227No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA990.6229941476307344No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA960.6041155370964697No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA960.6041155370964697No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA950.5978226669183815No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA930.585236926562205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA930.585236926562205No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA890.5600654458498522No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA870.5474797054936756No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA840.528601094959411No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA830.5223082247813228No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA810.5097224844251463No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG800.5034296142470581No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA710.44679378264426406No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA710.44679378264426406No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA710.44679378264426406No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA710.44679378264426406No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA680.4279151721099994No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA680.4279151721099994No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG660.4153294317538229No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG600.37757221068529356No Hit
CCTGTTCGATACCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCAGTG590.37127934050720535No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA580.36498647032911713No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTNAGCGTCAGTTGCGCTNCAGTG550.3461078597948524No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGNTGCGCTCCAGTG540.3398149896167642No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA540.3398149896167642No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA540.3398149896167642No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA530.333522119438676No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA530.333522119438676No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA520.3272292492605878No Hit
CCTGTTCGATACCCGCACTTTNGAGCTTCAGCGTCAGTTGCGCTCCAGTG510.3209363790824995No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG510.3209363790824995No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA470.29576489837014663No Hit
CCTGTTTGCTACCNACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA460.2894720281920584No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA450.28317915801397014No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA450.28317915801397014No Hit
CCTGTTTGCTACCCACGCTTTNGTGCCTCAGCGTCAGTTAAAGCCCAGCA440.27688628783588193No Hit
CCTGTTCGATACCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG440.27688628783588193No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA430.2705934176577937No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA430.2705934176577937No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA420.2643005474797055No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNNCGCC420.2643005474797055No Hit
CCTGTTCGANACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG420.2643005474797055No Hit
CCTGTTTGCTACCNACGCTTTCGTGCNTCAGNGTNAGTTAANGCNCAGCA410.2580076773016172No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA400.25171480712352906No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTTTNGTNCAGAA400.25171480712352906No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNCCGCC400.25171480712352906No Hit
CCTGTTTGCNACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA400.25171480712352906No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTNAGCGTCAGTTAAAGCNCAGCA400.25171480712352906No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGNTAAAGCCCAGCA390.24542193694544082No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGTTACGCTCCCGCC390.24542193694544082No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA390.24542193694544082No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTGCGGCA380.23912906676735257No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA370.23283619658926438No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA360.22654332641117614No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA350.2202504562330879No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA350.2202504562330879No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA350.2202504562330879No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA350.2202504562330879No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA350.2202504562330879No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGNGTCAGTTGCGCTNNAGTG350.2202504562330879No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA340.2139575860549997No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA340.2139575860549997No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGNGTCAGTTAAAGCNNAGCA330.20766471587691146No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCNCGCC320.20137184569882322No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA300.18878610534264678No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTAATNGTNCAGTA300.18878610534264678No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA300.18878610534264678No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTTTCGTCCAGAA300.18878610534264678No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA290.18249323516455856No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA290.18249323516455856No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA290.18249323516455856No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA280.17620036498647032No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA280.17620036498647032No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA280.17620036498647032No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA270.1699074948083821No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTAATCGTNCAGTA270.1699074948083821No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTAATCGTCCAGTA250.15732175445220567No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA240.15102888427411743No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA240.15102888427411743No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA240.15102888427411743No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTTGAGCGCCGGCA240.15102888427411743No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA240.15102888427411743No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA240.15102888427411743No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA220.13844314391794096No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA220.13844314391794096No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGNTACGCTCCCGCC220.13844314391794096No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA220.13844314391794096No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA210.13215027373985275No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA210.13215027373985275No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA200.12585740356176453No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA200.12585740356176453No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA200.12585740356176453No Hit
CCTGTTCGATACCNGCGCTTTCGAGCNTCAGNGTNAGTTACNCTNCCGCC200.12585740356176453No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGCA200.12585740356176453No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA190.11956453338367629No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA190.11956453338367629No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA190.11956453338367629No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA190.11956453338367629No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA180.11327166320558807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA180.11327166320558807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA180.11327166320558807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNAATCGTCCAGTA180.11327166320558807No Hit
CCTGTTCGANACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNCCGCC180.11327166320558807No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA180.11327166320558807No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGNGTCAGTTACGCTNNCGCC180.11327166320558807No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA170.10697879302749985No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG170.10697879302749985No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA170.10697879302749985No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTAATCGTNNAGTA170.10697879302749985No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTNCGGCA170.10697879302749985No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA170.10697879302749985No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG170.10697879302749985No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA170.10697879302749985No Hit
CCTGTTCGATACCNGCGCTTTCGAGCTTCAGCGTCAGTTACGCTNCCGCC170.10697879302749985No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA160.10068592284941161No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA160.10068592284941161No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA160.10068592284941161No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA160.10068592284941161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)