FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28356
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG422714.906898011002962No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA315411.12286641275215No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA292310.308224009028072No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA24048.477923543518125No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA18136.393708562561716No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA12374.362392438989985No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA11454.037946113697277No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA10703.773451826773875No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG7802.750740584003385No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC7742.7295810410495136No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA6042.1300606573564678No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTAGCGTGC5531.9502045422485543No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTAGCGTGA4571.611651854986599No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTCGCGTGA4061.4317957398786854No Hit
TGAGGAATATTGGTCAATGGGCGGGAGCCTGAACCAGCCAAGTAGCGTGA3931.3859500634786288No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA2810.9909719283396812No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA2620.9239667089857526No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGT2560.9028071660318804No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2200.7758499083086472No Hit
TGAGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCCATGCCGCGTGA2070.7300042319085908No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA1940.6841585555085344No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT1810.6383128791084779No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA1710.6030469741853576No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA1670.5889406122161095No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA1570.5536747072929891No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGATGCCGCGTGA1540.5430949358160531No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC1510.5325151643391169No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA1420.5007758499083087No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA1320.46550994498518833No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1320.46550994498518833No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA1300.45845676400056423No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA960.33855268726195514No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA960.33855268726195514No Hit
TTAGGAATATTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGA920.324446325292707No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCAACGCCGCGTGA890.3138665538157709No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC890.3138665538157709No Hit
TTAGGAATATTCGGCAATGGGGGAAACCCTGACCGAGCAATGCCGCGTGT840.29623360135421073No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA840.29623360135421073No Hit
TGGGGAATATTGCGCAATGGGGGGAACCCTGACGCAGCAACGCCGCGTGA780.27507405840033855No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA760.2680208774157145No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGT650.22922838200028212No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTCGCGTGA620.21864861052334605No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGA610.215122020031034No Hit
TAGGGAATCTTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGA510.1798561151079137No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGA500.17632952461560164No Hit
TGGGGGATATTGCACAATGGAGGGAACTCTGATGCAGCAACGCCGCGTGA480.16927634363097757No Hit
TGAGGAATATTGGTCAATGGGCGATGGCCTGAACCAGCCAAGTAGCGTGA480.16927634363097757No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGATGCCGCGTGA460.1622231626463535No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA460.1622231626463535No Hit
TGGGGAATATTGGGCAATGGGGGCAACCCTGACCCAGCAACGCCGCGTGA450.15869657215404148No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCGACGCCGCGTGA400.14106361969248132No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA350.12343066723092115No Hit
TGAGGGATATTGGTCAATGGGGGAAACCCTGAACCAGCAACGCCGCGTGA330.11637748624629708No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAATGCCGCGTGA320.11285089575398505No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA320.11285089575398505No Hit
ACTAATCCGTAACTGACGTTCATGCTCGAAAGTGTGGGTATCAAACAGGA290.10227112427704894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)