FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73244
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA28773.9279667959150237No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG13261.81038719895145No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA10691.4595052154442685No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA10651.4540440172573863No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA10001.3652995467205504No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA8181.1168150292174102No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA6980.9529790836109442No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6690.9133853967560482No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA6440.8792529080880345No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA5290.7222434602151712No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCGGTATCGGTCTGGCG4970.6785538747201136No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG4860.6635355797061876No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG4520.6171153951176889No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG4160.567964611435749No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4060.5543116159685435No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3970.5420239200480585No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA3890.5311015236742942No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA3580.4887772377259571No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA3570.48741193817923656No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA3480.47512424225875155No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3440.4696630440718694No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA3390.4628365463382666No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA3370.4601059472448255No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA3350.4573753481513844No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA3350.4573753481513844No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA3340.4560100486046639No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA3170.43279995631041446No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG3170.43279995631041446No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA3140.4287040576702528No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA3040.4150510622030474No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA3010.4109551635628857No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3000.40958986401616515No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG2950.40276336628256243No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA2870.39184096990879796No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2520.3440554857735787No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA2480.3385942875866965No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2390.32630659166621157No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2290.31265359619900607No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2280.3112882966522855No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA2260.30855769755884443No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGAA2230.30446179891868275No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA2170.2962700016383595No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA2150.29353940254491834No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCGGTATCGGTNTGGCG2140.2921741029981978No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCGGTATCGGTCNGGCG2110.28807820435803616No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA2060.2812517066244334No Hit
CCTGTTCGATACCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG2040.2785211075309923No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA1950.26623341161050734No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGAA1870.25531101523674293No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1860.2539457156900224No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCGGTATCGGTNNGGCG1800.2457539184096991No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA1800.2457539184096991No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1780.243023319316258No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGNTGCGCTCCAGTG1710.23346622248921414No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1680.22937032384905248No Hit
CCTGTTCGATACCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCAGTG1670.22800502430233194No Hit
CCTGTTCGATACCCGCACTTTNGAGCTTCAGCGTCAGTTGCGCTCCAGTG1640.2239091256621703No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1640.2239091256621703No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA1630.22254382611544976No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA1610.21981322702200862No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG1590.21708262792856753No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTNAGCGTCAGTTGCGCTNCAGTG1580.21571732838184696No Hit
CCTGTTCGANACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1580.21571732838184696No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA1560.21298672928840587No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA1550.21162142974168532No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGNGTCAGTTGCGCTNNAGTG1530.20889083064824424No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1530.20889083064824424No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA1480.2020643329146415No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1480.2020643329146415No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTNNAGAA1470.20069903336792094No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA1450.1979684342744798No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1450.1979684342744798No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA1430.19523783518103874No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1420.19387253563431817No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1400.19114193654087708No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGAA1360.18568073835399487No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1340.18295013926055376No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA1310.17885424062039212No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA1300.17748894107367158No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA1290.176123641526951No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG1250.1706624433400688No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA1240.16929714379334826No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA1240.16929714379334826No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCNAGAA1230.16793184424662772No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1220.16656654469990717No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA1210.16520124515318663No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTNGGTATCGGTCTGGCG1200.16383594560646608No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA1200.16383594560646608No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1190.1624706460597455No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1180.16110534651302494No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA1170.1597400469663044No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTNCAGGG1150.1570094478728633No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1140.15564414832614276No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCNAGGG1120.15291354923270167No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTNNAGGG1120.15291354923270167No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA1080.14745235104581944No Hit
CCTGTTTGCTCCCCACGCTCTCGCGCCTCAGCGTCAGTAATCGGCCAGAT1070.1460870514990989No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA1060.14472175195237835No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1040.14199115285893724No Hit
CCCGTTTGCTCCCNTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA1020.13926055376549615No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1020.13926055376549615No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA1010.1378952542187756No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA1010.1378952542187756No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTAACGCTCCGGAC1010.1378952542187756No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACAGTNCAGTA1000.13652995467205506No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACAGTCCAGTA1000.13652995467205506No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACAGTNNAGTA1000.13652995467205506No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA950.12970345693845228No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA950.12970345693845228No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA940.12833815739173174No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNNAGCA930.1269728578450112No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTACAGTCCAGAA930.1269728578450112No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA920.12560755829829065No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA920.12560755829829065No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA910.1242422587515701No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG900.12287695920484955No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACAGTCCAGTA900.12287695920484955No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA900.12287695920484955No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNCAGCA900.12287695920484955No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA890.121511659658129No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA880.12014636011140843No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA880.12014636011140843No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGNGTCGGTATCGGTNNGGCG860.11741576101796734No Hit
CCCGTTNGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA860.11741576101796734No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA850.11605046147124679No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA850.11605046147124679No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA850.11605046147124679No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC840.11468516192452624No Hit
CCCGTTNGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA830.1133198623778057No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA820.11195456283108515No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA800.10922396373764404No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTNAGNTGTCGTCCAGAA800.10922396373764404No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA790.10785866419092348No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCNAGCA770.10512806509748239No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA770.10512806509748239No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA770.10512806509748239No Hit
CCCGTTTGCNCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA760.10376276555076185No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG760.10376276555076185No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA760.10376276555076185No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGNGTCAGTAGCAGTNNAGTA760.10376276555076185No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG740.10103216645732074No Hit
CCCGTTTGCTCCCNTGGCTTTCGCGCNTCAGNGTNAGTTGTNGTNCAGAA740.10103216645732074No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA740.10103216645732074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)