FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60539
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA22893.7810337138043244No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA11031.8219660053849582No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA8661.4304828292505658No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA8641.4271791737557609No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA8221.3578024083648559No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA7881.3016402649531706No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA6161.0175258923999406No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA5850.966319232230463No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT5430.896942466839558No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA5250.8672095673863129No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4900.8093955962272255No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA4460.7367151753415154No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA4210.695419481656453No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA4160.6871603429194404No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA3840.6343018550025603No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGCTTGGTG3550.5863988503278879No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA3060.5054592907051653No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA3060.5054592907051653No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2990.49389649647334777No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2940.4856373577363352No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2910.4806818744941277No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA2910.4806818744941277No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA2730.4509489750408827No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2700.4459934917986752No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2700.4459934917986752No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2670.4410380085564677No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA2630.4344306975668577No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2610.43112704207205266No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2600.4294752143246502No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2540.41956424784023516No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA2530.4179124200928327No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2510.4146087645980277No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA2430.4013941426188077No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA2400.39643865937660017No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2400.39643865937660017No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA2320.3832240373973802No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2290.3782685541551727No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA2210.3650539321759527No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA2180.3600984489337452No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT2170.3584466211863427No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT2140.3534911379441352No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT2100.3468838269545252No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA2040.3369728604701102No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1970.32541006623829266No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA1950.32210641074348767No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA1930.3188027552486827No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA1910.3154990997538777No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1900.31384727200647516No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA1900.31384727200647516No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1860.3072399610168652No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1850.30558813326946266No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1820.30063265002725514No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA1600.26429243958440013No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1580.26098878408959514No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA1570.2593369563421926No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1520.2510778176051801No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1520.2510778176051801No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1510.24942598985777764No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA1410.23290771238375263No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1410.23290771238375263No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1380.22795222914154512No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGTTACAGTCCAGTT1310.21638943490972762No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1310.21638943490972762No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGTCCAGTA1310.21638943490972762No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1310.21638943490972762No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1260.2081302961727151No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1250.2064784684253126No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1250.2064784684253126No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1220.2015229851831051No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG1180.1949156741934951No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1180.1949156741934951No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGCNNGGTG1180.1949156741934951No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1170.1932638464460926No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGCTNGGTG1130.1866565354564826No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1120.1850047077090801No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGCNTGGTG1110.1833528799616776No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1090.1800492244668726No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1080.1783973967194701No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNNAGAA1070.1767455689720676No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG1050.1734419134772626No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1020.1684864302350551No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG1010.16683460248765258No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1000.1651827747402501No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA1000.1651827747402501No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1000.1651827747402501No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCNAGAA990.1635309469928476No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA990.1635309469928476No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA980.1618791192454451No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA980.1618791192454451No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA980.1618791192454451No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNNAGTA980.1618791192454451No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA970.1602272914980426No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGTG960.15857546375064008No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA950.15692363600323758No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA950.15692363600323758No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGTG940.1552718082558351No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA940.1552718082558351No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA930.1536199805084326No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG910.15031632501362757No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA910.15031632501362757No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA910.15031632501362757No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA900.14866449726622508No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNTAGTA890.14701266951882258No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA890.14701266951882258No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNTAGTA870.1437090140240176No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA860.14205718627661507No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA850.14040535852921257No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA850.14040535852921257No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTNAGTA850.14040535852921257No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTNAGTA840.13875353078181007No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAGTNNAGCA830.13710170303440758No Hit
CCTGTTNGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT820.13544987528700508No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGNTACAGTCCAGTT820.13544987528700508No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA820.13544987528700508No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGAA810.13379804753960256No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCCAGTG800.13214621979220006No Hit
CCTGTTTGCTCCCNACACTTTCGCGCNTCAGNGTNAGTTACNGTNCAGTT790.13049439204479757No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA790.13049439204479757No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA780.12884256429739507No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA780.12884256429739507No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTNAGTTACAGCTTGGTG770.12719073654999258No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAGTCCAGCA770.12719073654999258No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA760.12553890880259005No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA760.12553890880259005No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA760.12553890880259005No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNCAGTT760.12553890880259005No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNNAGTA750.12388708105518756No Hit
CCTGTTNGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT750.12388708105518756No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTNAGCGTCAGTTACAGTNCAGTT750.12388708105518756No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA740.12223525330778506No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA740.12223525330778506No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA740.12223525330778506No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA720.11893159781298007No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA710.11727977006557755No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGNGTCAGTTACAGTNNAGTT710.11727977006557755No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA700.11562794231817505No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA690.11397611457077256No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA690.11397611457077256No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGCA680.11232428682337006No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG680.11232428682337006No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA680.11232428682337006No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA680.11232428682337006No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA670.11067245907596757No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA660.10902063132856504No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA660.10902063132856504No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA660.10902063132856504No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTNAGTTATGGTTTAGTA640.10571697583376005No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCNTAGCA640.10571697583376005No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTNAGTTACAGGTTAGTA640.10571697583376005No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA620.10241332033895506No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCNAGTT620.10241332033895506No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNNAGTT610.10076149259155255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)