FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8168
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA6648.12928501469148No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTNNAGTA3233.954456415279138No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTNCAGTA2773.39128305582762No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCNAGTA2653.244368266405485No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCCAGAA1772.1669931439764936No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACAGTCCAGTA1601.9588638589618024No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1161.4201762977473065No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAANGTCAGTTACAGTNNAGTA1111.3589618021547503No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTNCAGTA991.212047012732615No Hit
CCTGTTTGCTCCCNACACTTTCGTGCNTCAANGTNAGTTACNGTNCAGTA891.0896180215475024No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNNAGAA831.016160626836435No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACAGTCCAGTA821.0039177277179236No Hit
CCTGTTTGCTCCCNACACTTTCGTGCCTCAACGTCAGTTACAGTNCAGTA780.9549461312438785No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNCAGAA760.930460333006856No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA740.9059745347698335No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTNAACGTCAGTTACAGTNCAGTA700.8570029382957884No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGNTACAGTCCAGTA660.8080313418217433No Hit
CCTGTTTGCNCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTNCAGTA650.7957884427032321No Hit
CCTGTTTGCTCCCCACACTTTNGTGCCTCAACGTCAGTTACAGTCCAGTA640.7835455435847208No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA570.697845249755142No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA510.6243878550440745No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCNAGAA510.6243878550440745No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTT420.514201762977473No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT410.5019588638589618No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTNAGTGTCAGTCCAGAA370.45298726738491674No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA370.45298726738491674No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA340.416258570029383No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA330.40401567091087165No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG310.3795298726738492No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTT300.3672869735553379No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA300.3672869735553379No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA280.3428011753183154No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA250.3060724779627816No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTNAGNGTCAGTCCAGAA250.3060724779627816No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG230.28158667972575907No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCCAGAA230.28158667972575907No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNCAGAA230.28158667972575907No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGNGTCAGTGTCAGTNNAGAA230.28158667972575907No Hit
CCTGTTTGCNCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNCAGAA220.2693437806072478No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA210.2571008814887365No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA200.2448579823702253No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA200.2448579823702253No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNCAGAA200.2448579823702253No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGTT200.2448579823702253No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA180.22037218413320275No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTNAGTGTCAGTCCAGAA180.22037218413320275No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA180.22037218413320275No Hit
CCTGTTTGCTCCCCACGCTTTNGTGCCTCAGCGTCAGTGTCAGTCCAGAA180.22037218413320275No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCNNAGAG170.2081292850146915No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA170.2081292850146915No Hit
CCCGTTCGCTGCCCTGGCTTTCGCACATGAGCGTCAGTTGTGTGCCAGGA170.2081292850146915No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTATCGTCCAGTA170.2081292850146915No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTA150.18364348677766895No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA140.1714005876591577No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA140.1714005876591577No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG140.1714005876591577No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNNAGTA140.1714005876591577No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCNTCAGNGTNAGTGTCNGTNCAGAA140.1714005876591577No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA140.1714005876591577No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA140.1714005876591577No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA140.1714005876591577No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT140.1714005876591577No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCNAGTA130.15915768854064644No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA130.15915768854064644No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT130.15915768854064644No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG120.14691478942213515No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTNAGCGTCAGTGTCAGTNCAGAA120.14691478942213515No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA120.14691478942213515No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA120.14691478942213515No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCNAGAG120.14691478942213515No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT120.14691478942213515No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCNAGCA110.1346718903036239No Hit
CCTGTTTGCTCCCNACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT110.1346718903036239No Hit
CNTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGTT110.1346718903036239No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCNCAGAG110.1346718903036239No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA110.1346718903036239No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTAAAAGCCCAGCA110.1346718903036239No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACCGTNNAGTA110.1346718903036239No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA100.12242899118511265No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA100.12242899118511265No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA100.12242899118511265No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA100.12242899118511265No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA100.12242899118511265No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA100.12242899118511265No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGNTAAAGCCCAGTT100.12242899118511265No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA100.12242899118511265No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAAAGCCCAGTT100.12242899118511265No Hit
CCTGTTTGCTCCCNACACTTTCGAGCNTCAGNGTNAGTTAANGCNCAGTT100.12242899118511265No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACCGTNCAGTA100.12242899118511265No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA90.11018609206660138No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA90.11018609206660138No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACCGTCCAGTA90.11018609206660138No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA90.11018609206660138No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNCAGTA90.11018609206660138No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA90.11018609206660138No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTGACGGCCCAGAG90.11018609206660138No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA90.11018609206660138No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA90.11018609206660138No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA90.11018609206660138No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA90.11018609206660138No Hit
CCTGTTTGCNCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT90.11018609206660138No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA90.11018609206660138No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTAATGTCCAGCA90.11018609206660138No Hit
CCTGTTTGCGCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA90.11018609206660138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)