FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37737
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA9782.5916209555608556No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA9112.4140763706706947No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG7792.0642870392453028No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA7602.013938574873466No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA5021.330259427087474No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3941.0440681559212444No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA3781.001669449081803No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA3540.938071388822641No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNNAGCA3460.9168720354029202No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCNAGCA3270.8665235710310835No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA3190.8453242176113629No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA2940.7790762381747357No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG2920.7737763998198055No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA2900.7684765614648753No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2880.7631767231099451No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG2580.6836791477859925No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA2570.6810292286085274No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA2460.6518801176564115No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG2440.6465802793014813No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA2400.6359806025916209No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2370.6280308450592257No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG2260.5988817341071098No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA2160.5723825423324589No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1990.5273339163155524No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA1950.516734239605692No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1890.5008347245409015No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA1850.4902350478310412No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1840.48758512865357606No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1820.48228529029864586No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA1720.455786098523995No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1440.38158836155497255No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1380.3656888464901821No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1330.35243925060285664No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA1260.333889816360601No Hit
CCTGTTTGCTCCCNACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA1260.333889816360601No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGNGTCAGTTAGAGCNNAGCA1260.333889816360601No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA1240.3285899780056708No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA1230.32594005882820576No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA1230.32594005882820576No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA1220.32329013965074066No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1180.3126904629408803No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1180.3126904629408803No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1180.3126904629408803No Hit
CCTGTTCGATACCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1170.3100405437634152No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGNTGCGCTCCAGTG1160.3073906245859501No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA1140.30209078623102No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1140.30209078623102No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1130.2994408670535549No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTNAGCGTCAGTTAGAGCNCAGCA1130.2994408670535549No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA1110.2941410286986247No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1090.2888411903436945No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1070.28354135198876435No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG1060.28089143281129925No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA1040.2755915944563691No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAGAGCCCAGCA1040.2755915944563691No Hit
CCTGTTCGANACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG1040.2755915944563691No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1030.272941675278904No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA1030.272941675278904No Hit
CCTGTTTGCTCCCNACACTTTCGAGCNTCAGNGTNAGTTAGNGCNCAGCA1020.2702917561014389No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1010.2676418369239738No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA1010.2676418369239738No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGNTAGAGCCCAGCA1000.26499191774650876No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1000.26499191774650876No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA990.26234199856904367No Hit
CNTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA970.25704216021411347No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA970.25704216021411347No Hit
CCTGTTTGCNCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA960.25439224103664837No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA960.25439224103664837No Hit
CCTGTTCGATACCCGCACTTTNGAGCTTCAGCGTCAGTTGCGCTCCAGTG960.25439224103664837No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCACCGTCAGTTGTCGTCCAGCA960.25439224103664837No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA960.25439224103664837No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGNTGTCGTCCAGCA950.25174232185918327No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA930.2464424835042531No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA920.24379256432678803No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA920.24379256432678803No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA910.24114264514932293No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG900.23849272597185783No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA890.2358428067943928No Hit
CCTGTTCGATACCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCAGTG880.2331928876169277No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG880.2331928876169277No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA880.2331928876169277No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGNGTCAGTTGCGCTNNAGTG870.2305429684394626No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTNAGCGTCAGTTGCGCTNCAGTG850.22524313008453245No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNACCGTCAGTTGTCGTNCAGCA810.21464345337467208No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG800.21199353419720698No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCACNGTNAGTTGTNGTNCAGCA800.21199353419720698No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA790.20934361501974189No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA780.2066936958422768No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA760.20139385748734664No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA730.19344409995495138No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA720.19079418077748628No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA700.1854943424225561No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG690.18284442324509104No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA680.18019450406762594No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA670.17754458489016087No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA670.17754458489016087No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA660.17489466571269577No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA660.17489466571269577No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA640.16959482735776557No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA630.1669449081803005No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT620.1642949890028354No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA620.1642949890028354No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA620.1642949890028354No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA610.16164506982537033No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA590.15634523147044016No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG590.15634523147044016No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNNAGTA580.15369531229297506No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA580.15369531229297506No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA570.15104539311551No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA570.15104539311551No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA550.1457455547605798No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA550.1457455547605798No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA550.1457455547605798No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA550.1457455547605798No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA540.14309563558311472No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA530.14044571640564962No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG520.13779579722818455No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA520.13779579722818455No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA520.13779579722818455No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA510.13514587805071945No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCNAGTA510.13514587805071945No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA510.13514587805071945No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA500.13249595887325438No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA490.12984603969578928No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA490.12984603969578928No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA490.12984603969578928No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA490.12984603969578928No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA480.12719612051832418No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA460.12189628216339402No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA440.11659644380846385No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAGTCCAGCA440.11659644380846385No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTNCAGCA440.11659644380846385No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG440.11659644380846385No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTGTCGTNNAGCA430.11394652463099875No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA420.11129660545353368No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGTG420.11129660545353368No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA420.11129660545353368No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA410.10864668627606858No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA410.10864668627606858No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCNNAGCA410.10864668627606858No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTGTCGTNCAGCA400.10599676709860349No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGANTTGTA400.10599676709860349No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA390.1033468479211384No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA390.1033468479211384No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA380.10069692874367332No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA380.10069692874367332No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTGTNGTNCAGCA380.10069692874367332No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA380.10069692874367332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)