FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53207
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA14472.7195669742703026No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA14412.7082902625594376No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA13712.576728625932678No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA7171.347567049448381No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA6891.294942394797677No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA6051.1370684308455654No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA5641.0600109008213203No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA5621.0562519969176989No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA5150.9679177551825888No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA5130.9641588512789672No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA4980.9359670720018042No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA4960.9322081680981826No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA4790.9002574849173981No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT4480.8419944744112617No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA4460.83823557050764No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA4180.7856109158569361No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3760.7066739338808804No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3500.6578081831337982No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA3400.6390136636156897No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3360.6314958558084462No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA3170.5957862687240401No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA2870.5394027101697145No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA2740.5149698347961734No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2720.5112109308925518No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA2680.5036931230853083No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2630.494295863326254No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA2560.48113969966357806No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA2530.47550134380814557No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2480.4661040840490913No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA2290.4303944969646851No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2060.3871671020730355No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2010.37776984231398125No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1990.37401093841035954No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1950.36649313060311617No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1910.35897532279587274No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1910.35897532279587274No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1850.3476986110850076No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT1790.3364218993741425No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNNAGAA1790.3364218993741425No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1780.33454244742233163No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1750.3289040915668991No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA1750.3289040915668991No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1750.3289040915668991No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1730.3251451876632774No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1730.3251451876632774No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1720.32326573571146655No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA1690.31762737985603395No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT1690.31762737985603395No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1670.3138684759524123No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA1670.3138684759524123No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA1670.3138684759524123No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1650.3101095720487906No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1630.30635066814516887No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCNAGAA1620.304471216193358No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1610.30259176424154716No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1600.3007123122897363No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1580.2969534083861146No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1560.2931945044824929No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1550.291315052530682No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1550.291315052530682No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA1540.2894356005788712No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1530.28755614862706036No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1510.28379724472343865No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1480.27815888886800605No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA1460.2743999849643844No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1460.2743999849643844No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1460.2743999849643844No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA1440.2706410810607627No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1440.2706410810607627No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1410.2650027252053301No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA1390.2612438213017084No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT1370.2574849173980867No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1340.25184656154265417No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA1250.23493149397635651No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1200.2255342342173022No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1150.21613697445824798No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1150.21613697445824798No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1130.21237807055462626No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTNNAGTA1100.20673971469919372No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1090.20486026274738287No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA1060.19922190689195032No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTNCAGTA1060.19922190689195032No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGTTACAGTCCAGTT1050.19734245494013947No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA1040.1954630029883286No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG1040.1954630029883286No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTTTCGTCCAGAA1040.1954630029883286No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1010.18982464713289604No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1000.1879451951810852No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA970.18230683932565264No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA970.18230683932565264No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA970.18230683932565264No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA960.18042738737384179No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTCNAGTA950.17854793542203093No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA950.17854793542203093No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA950.17854793542203093No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA940.1766684834702201No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA940.1766684834702201No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA930.17478903151840924No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGTA920.1729095795665984No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA920.1729095795665984No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA920.1729095795665984No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA910.17103012761478753No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG900.16915067566297667No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA870.16351231980754413No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA860.16163286785573328No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA830.1559945120003007No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA830.1559945120003007No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA820.15411506004848985No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA810.152235608096679No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA780.14659725224124645No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA780.14659725224124645No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA780.14659725224124645No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA760.14283834833762477No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA750.14095889638581388No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA750.14095889638581388No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG750.14095889638581388No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGTA730.1371999924821922No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA720.13532054053038134No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA700.13156163662675965No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA690.1296821846749488No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA690.1296821846749488No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTNAGTTACAGTCCAGTA690.1296821846749488No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA680.12780273272313794No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA680.12780273272313794No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGTA670.12592328077132708No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG670.12592328077132708No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA660.12404382881951624No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA650.12216437686770537No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGNTACAGTCCAGTT650.12216437686770537No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA650.12216437686770537No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTTTNGTNCAGAA630.11840547296408369No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA620.11652602101227283No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTNAACGTCAGTTACTGTNCAGAA590.11088766515684027No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGTA590.11088766515684027No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA570.10712876125321856No Hit
CCTGTTTGCTCCCNACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT570.10712876125321856No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGNTACTGTCCAGAA570.10712876125321856No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA570.10712876125321856No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA570.10712876125321856No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGNGTCAGTTACAGTNNAGTT560.10524930930140772No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA550.10336985734959686No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA550.10336985734959686No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA540.101490405397786No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA540.101490405397786No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA540.101490405397786No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA540.101490405397786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)