FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46783
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA14743.1507171408417585No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA11612.48167069234551No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA10242.1888292755915613No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG10182.1760041040548916No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA8611.840412115512045No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA6151.3145800825086036No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA5211.1136523951007844No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG5131.0965521663852253No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA4921.0516640660068828No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA4811.0281512515229891No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG4721.0089134942179852No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA4380.9362375221768591No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA4200.8977620075668512No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA4170.8913494217985165No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG4030.8614240215462883No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA4030.8614240215462883No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA4010.8571489643673984No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG3840.8208109783468355No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA3820.8165359211679456No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA3790.8101233353996109No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA3780.8079858068101661No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3720.7951606352734968No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG3670.7844729923262723No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCNAGTA3530.754547592074044No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTNNAGTA3300.7053844345168117No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTNCAGTA2940.6284334052967958No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCTAGTG2570.5493448474873351No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA2470.5279695615928862No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACTGTCCAGTA2340.5001816899301028No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA2260.48308146121454376No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA2260.48308146121454376No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA2190.46811876108842954No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTATCGTCCAGTA2090.4467434751939807No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA2050.4381933608362012No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA2020.4317807750678665No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA1980.423230660710087No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1970.4210931321206421No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1930.41254301776286256No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA1920.41040548917341774No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1910.4082679605839728No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA1890.40399290340508304No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1850.39544278904730346No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1840.39330526045785863No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAGTCCAGCA1810.386892674689524No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA1770.3783425603317444No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1690.36124233161618535No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTNCAGGG1680.35910480302674047No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTGCAGTNNAGTG1660.3548297458478507No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAGTNNAGCA1630.34841716007951606No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTNNAGGG1600.3420045743111814No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA1600.3420045743111814No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA1590.33986704572173654No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA1570.3355919885428468No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA1500.32062928841673255No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1500.32062928841673255No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA1490.31849175982728767No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTAATCGTNNAGTA1480.3163542312378428No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1450.3099416454695082No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1450.3099416454695082No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1440.30780411688006326No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNAATCGTCCAGTA1430.3056665882906184No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1420.3035290597011735No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACTGTCCAGTA1410.3013915311117286No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1410.3013915311117286No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTAATNGTNCAGTA1390.29711647393283885No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACTGTNNAGTA1380.294978945343394No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCNAGGG1370.2928414167539491No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACTGTCCAGTA1330.2842913023961695No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA1320.28215377380672463No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG1300.2778787166278349No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTAATCGTCCAGTA1290.27574118803839004No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1290.27574118803839004No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTAATCGTNCAGTA1280.27360365944894516No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG1280.27360365944894516No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGTGTCAGTTGCAGTCTAGTG1270.2714661308595003No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACTGTNCAGTA1260.2693286022700554No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTGCNGTNTAGTG1240.2650535450911656No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1240.2650535450911656No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTATNGTNCAGTA1230.2629160165017207No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA1190.25436590214394117No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCCAGTG1180.2522283735544963No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGNTGCAGTCTAGTG1180.2522283735544963No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTATCGTNNAGTA1170.2500908449650514No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA1160.2479533163756065No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCTAGTG1140.24367825919671676No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1140.24367825919671676No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTATCGTNCAGTA1110.23726567342838212No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGTGTCAGTTGCAGTNTAGTG1110.23726567342838212No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA1110.23726567342838212No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1110.23726567342838212No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACTGTCCAGTA1100.23512814483893724No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG1070.22871555907060256No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTATCGTNCAGTA1030.220165444712823No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTNCAGTA1030.220165444712823No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTNCAGTA1010.21589038753393325No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTATCGTCCAGTA1010.21589038753393325No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTATCGTCCAGTA1000.21375285894448837No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG990.2116153303550435No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG980.20947780176559863No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTNAGTCTTCGTCCAGGG970.20734027317615375No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTATCGTCCAGTA970.20734027317615375No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA890.19024004446059464No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA850.18168993010281512No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA830.17741487292392535No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCNTGGCA830.17741487292392535No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCNNGGCA830.17741487292392535No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGAA820.17527734433448047No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA810.1731398157450356No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA790.16886475856614583No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA790.16886475856614583No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA790.16886475856614583No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA790.16886475856614583No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA790.16886475856614583No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCNAGCA790.16886475856614583No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA760.16245217279781116No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTNNAGCA750.16031464420836627No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTNGGCA750.16031464420836627No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA750.16031464420836627No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTTTCGTCCAGAA730.1560395870294765No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA730.1560395870294765No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTTTNGTNCAGAA730.1560395870294765No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGAA720.15390205844003163No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTNCAGCA690.147489472671697No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGNTATAGTTTGGTA690.147489472671697No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA670.14321441549280722No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA650.13893935831391746No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTNAGNCTTCGTCCAGGG650.13893935831391746No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG640.13680182972447258No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGTGTCAGTTGCAGTNCAGTG630.1346643011350277No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA630.1346643011350277No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA630.1346643011350277No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG630.1346643011350277No Hit
CCTGTTTGCNCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTNCAGGG630.1346643011350277No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA620.1325267725455828No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA610.1303892439561379No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTNNAGCA610.1303892439561379No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA610.1303892439561379No Hit
CCTGTTTGCTCCCNACGCTTTCGCACCTGAGCGTCAGTCTTCGTNCAGGG590.12611418677724814No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT580.12397665818780325No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTGCNGTNCAGTG560.1197016010089135No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCCAGTG550.11756407241946862No Hit
CCTGTTTGCTCCCCACGCTTTNGCACCTGAGCGTCAGTCTTCGTCCAGGG540.11542654383002372No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA540.11542654383002372No Hit
CCTGTTTGCNCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA540.11542654383002372No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTNAGTCATGGCTTGGCA520.11115148665113396No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG520.11115148665113396No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA500.10687642947224418No Hit
CCTGTTTGCTCCCNACGCTTTCGCACNTGAGNGTNAGTCTTNGTNCAGGG500.10687642947224418No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTNAGCGTCAGTCTTCGTNCAGGG500.10687642947224418No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGNGTCAGTCTTCGTNNAGGG490.10473890088279932No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA490.10473890088279932No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA490.10473890088279932No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA490.10473890088279932No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA480.10260137229335443No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCNAGCA480.10260137229335443No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA470.10046384370390954No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA470.10046384370390954No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTNAACGTCAGATATAGTNTGGTA470.10046384370390954No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGNTGCAGTCCAGTG470.10046384370390954No Hit
CCTGTTNGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG470.10046384370390954No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA470.10046384370390954No Hit
CNTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTNAGTCTTCGTCCAGGG470.10046384370390954No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAANGTCAGATATAGTNNGGTA470.10046384370390954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)