FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59412
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA890814.99360398572679No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA675711.373123274759307No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA51118.602639197468525No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA21133.5565205682353733No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA17512.9472160506295024No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA16012.6947418030027603No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA15952.6846428330976906No Hit
CNCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA12112.038308759173231No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG10291.7319733387194507No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG8531.43573688817074No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA7331.2337574900693462No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA6791.1428667609237193No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG5680.9560358176799301No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3970.6682151753854441No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3960.6665320137345991No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3910.6581162054803744No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA3660.6160371642092506No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA3310.5571265064296775No Hit
NNTGTTTGNTANCCACACTTTCGAGCATCNGCGTCAGTTACAGTCCAGTA2720.4578199690298256No Hit
CNTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG2520.42415673601292664No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA2480.4174240894095469No Hit
NNTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2400.4039587962027873No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2320.39049350299602775No Hit
NNTGTTTGNTANCCACGCTTTCGTGCCTCNGCGTCAGTTAAAGCCCAGCA2140.3601965932808187No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2000.33663233016898947No Hit
NNTGTTTGCTACCNACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1930.3248501986130748No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGTA1860.31306806705716017No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1830.3080185821046253No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG1740.2928701272470208No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1700.28613748064364103No Hit
NNCGTTTGNTCNCCTGGCTTTCGCGCCTCNGCGTCAGTTGTCGTCCAGAA1580.26593954083350163No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGTA1540.2592068942301219No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1490.25079108597589717No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCTAGTA1470.24742476267420724No Hit
CNTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1340.2255436612132229No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA1200.20197939810139365No Hit
NNCGTTTGCTCCCNTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1060.1784151349895644No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGTCCAGCA940.15821719517942504No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA920.15485087187773514No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA910.15316771022689019No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCTCAGCA860.14475190197266546No Hit
CCTGTTTGATACACACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA860.14475190197266546No Hit
CNTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA840.14138557867097556No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAACGCCCAGCA790.13296977041675082No Hit
CNCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA790.13296977041675082No Hit
GCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA790.13296977041675082No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA760.127920285464216No Hit
CNCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGTA750.12623712381337104No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAATCCCAGCA710.11950447720999126No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG710.11950447720999126No Hit
CNTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA710.11950447720999126No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCTAGCA710.11950447720999126No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCTGTA690.11613815390830134No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAC640.10772234565407661No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA610.10267286070154177No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA600.10098969905069682No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACCGTCCAGTA600.10098969905069682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)