FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002449463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103648
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA97319.388507255325717No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG60285.815838221673356No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA33343.216656375424514No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA25752.484370175980241No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA16911.6314834825563445No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA16701.6112225995677676No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA13931.3439719049089225No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA10811.0429530719357827No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA9450.9117397344859524No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA9180.8856900277863539No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG5680.5480086446434085No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA5670.5470438406915715No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG5460.5267829577029948No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA5110.49301481938870023No Hit
CCTGTTTGATACCCACACTCTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3920.3782031491200988No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3920.3782031491200988No Hit
CCTGTTTGATACCCACACTCTCGAGCATGAACGTCAGTTACGGATTAGTG3580.34539981475764125No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA3320.3203149120098796No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG3270.31549089225069465No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA3150.3039132448286508No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG2590.24988422352577958No Hit
CCTGTTTGATACCCACACTTTCGAGCATGCACGTCAGTTACGGATTAGTG2580.24891941957394256No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2570.2479546156221056No Hit
CCTGTTTGATACCCACACTTTCGCGCATGAACGTCAGTTACGGATTAGTG2550.2460250077184316No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG2430.23444736029638777No Hit
CCTGTTTGATACACACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2400.23155294844087682No Hit
ACTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2400.23155294844087682No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT2370.22865853658536583No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT2340.22576412472985488No Hit
CCTATTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2330.22479932077801792No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA2290.22094010497066993No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCCATGTCAGTTGCAGCTTAGCA2250.21708088916332202No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA2250.21708088916332202No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA2160.2083976535967891No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA2140.20646804569311514No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA2130.20550324174127818No Hit
CCTGTTTGATACCCACACTTTCGAGCCTGAACGTCAGTTACGGATTAGTG2100.20260882988576723No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2050.19778481012658228No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACGGATTAGTG2010.1939255943192343No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2000.19296079036739733No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1990.19199598641556037No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1850.17848873108984253No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1810.1746295152824946No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGCA1780.17173510342698364No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1720.1659462797159617No Hit
CCTGTTTGATACACACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG1540.14857980858289596No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1450.13989657301636307No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTA1390.13410774930534114No Hit
CCCGTTTGCTACCCTGGCTCTCGCATCTCAGCGTCAGACACAGTCCAGAA1390.13410774930534114No Hit
ACTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG1380.13314294535350416No Hit
CCTATTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG1350.13024853349799323No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTTAGCA1350.13024853349799323No Hit
CCTGTTTGATACCCACACCTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1240.11963569002778635No Hit
CCCGTTTGCTACCCTCGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1220.11770608212411239No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGCA1220.11770608212411239No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA1160.11191725841309046No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1150.11095245446125347No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTCGCA1140.10998765050941649No Hit
CCTGTTTGATACCCACACCTTCGAGCATGAACGTCAGTTACGGATTAGTG1050.10130441494288361No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT1040.10033961099104663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)