FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002449487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78325
Sequences flagged as poor quality0
Sequence length301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA51886.623683370571337No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTTAGCA24943.184168528566869No Hit
CCCGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGAGACAGCCCAGAG22762.905841046919885No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20592.6287902968400894No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGCA16132.059368017874242No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA13111.6737950845834664No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA12511.5971911905521865No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTCAATCGCGGTTTCGTG8741.115863389722311No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTTAGCA8311.060963932333227No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA8301.059687200766039No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA7981.018831790616023No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTGCGGCG5430.6932652409830833No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA5210.6651771465049474No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA5050.6447494414299393No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG3950.5043089690392595No Hit
CCTGTTCGATACCCACGCCTTCGAGCTTCAGCGTCAGTGCGGCGCTGGCA3500.4468560485157995No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTGCAGCTTAGCA3080.3932333226939036No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGTGTCAATATATAGCCAGAT3010.3842962017235876No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2980.38046600702202366No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC2900.3702521544845196No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA2870.36642195978295566No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2780.3549313756782636No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA2590.33067347590169166No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2420.3089690392594957No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2350.30003191828917974No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2180.27832748164698373No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA2130.27194382381104376No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA2110.2693903606766677No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA2020.25789977657197577No Hit
CCTCTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1940.24768592403447173No Hit
CCCGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGAGACAGCCCAGCG1860.23747207149696775No Hit
CCTGTTTGATACCCACACTTTCGAGCTTCAATGTCAGTTACAGTTTAGTG1560.1991701244813278No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG1470.1876795403766358No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1320.16852856686881584No Hit
CCTGTTTGCTACCCACACCTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1290.16469837216725183No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1230.15703798276412384No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAACGTCAGTTGCAGCTTAGCA1140.14554739865943184No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGAACGAGCCTGGGA1130.14427066709224384No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCCGTTGGTGCCCAGTA1100.14044047239067986No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGCA1070.13661027768911588No Hit
CCTGTTTGATACCCACACTCTCGAGCCTCAACGTCAGTTGCAGCTTAGCA1010.12894988828598788No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCCGCGTCAGTTGGTGCCCAGTA1010.12894988828598788No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGCTGCCCAGTA1010.12894988828598788No Hit
CCCGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGAGACAGCCCCGAG1000.12767315671879986No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT990.12639642515161187No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCTAGCA960.12256623045004789No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA960.12256623045004789No Hit
CCTGTTTGCTACCCATGCTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA930.11873603574848388No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTGCGGCCTAGCA910.11618257261410789No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA910.11618257261410789No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA900.1149058410469199No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGCA880.11235237791254388No Hit
CCCGTTCGCTACCCACGCTCTCGCTCCTCAGCGTCAGAGACAGCCCAGAG860.1097989147781679No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCCACGTCAGTTGCAGCTTAGCA810.1034152569422279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)