FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51036
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA563911.049063406223059No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA25625.01998589231131No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA21874.285210439689631No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA18093.544556783446979No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA13062.5589779763304334No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12852.5178305509836196No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTACCAGTCCAGTA9221.8065679128458343No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA9151.7928521043968964No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA8011.569480366799906No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG6801.3323928207539777No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA6131.2011129398855709No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT5701.1168586879849518No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG5501.077670663845129No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA5391.0561172505682264No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG4970.9738223998745983No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4850.9503095853907045No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA4620.9052433576299084No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA4380.8582177286621209No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA4140.8111920996943334No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA4080.7994356924523864No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3600.7053844345168117No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA3230.6328865898581394No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG3200.6270083862371659No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2990.585860960890352No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2830.5545105415784936No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2630.5153225174386707No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA2300.450662277607963No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA2210.4330276667450427No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2190.42910886433106044No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTACCAGTCCAGTA2180.4271494631240693No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA2170.4251900619170782No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA1970.3860020377772553No Hit
NNTGTTTGNTANCCACACTTTCGAGCCTCNGCGTCAGTTGGTGCCCAGTA1950.382083235363273No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1930.3781644329492907No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTATTGGCCTAGTG1860.36444862450035265No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA1820.3566110196723881No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1800.3526922172584058No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA1800.3526922172584058No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1770.3468140136374324No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA1550.30370718708362726No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGTCCAGTA1530.29978838466964497No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1490.2919507798416804No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1480.28999137863468927No Hit
CNTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1470.2880319774276981No Hit
NNTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1430.2801943725997335No Hit
CNTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1270.24884395328787523No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1220.2390469472529195No Hit
CNCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1100.2155341327690258No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1100.2155341327690258No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAACTTAGCA1090.21357473156203466No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTTCCCAGTA1080.2116153303550435No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1060.20769652794106122No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1060.20769652794106122No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1010.1978995219061055No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG990.19398071949212323No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA950.18614311466415862No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA950.18614311466415862No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA940.18418371345716747No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA880.17242730621522062No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG870.17046790500822948No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA860.16850850380123833No Hit
NNTGTTTGNTCNCCACGCTTTCGAGCCTCNACGTCAGTTACAGTCCAGTA850.1665491025942472No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA840.16458970138725607No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG840.16458970138725607No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA820.16067089897327377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA810.15871149776628263No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA800.15675209655929148No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA800.15675209655929148No Hit
NNTGTTTGNTANCCACACTTTCGAGCCTCNATGTCAGTTGCAGCTTAGCA780.1528332941453092No Hit
CNTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA760.14891449173132693No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA700.13715808448938005No Hit
NNTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA690.1351986832823889No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA690.1351986832823889No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCGTAGCA660.12932047966141547No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTACAGTCCAGCA650.12736107845442432No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA640.12540167724743317No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA620.1214828748334509No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG590.11560467121247747No Hit
NNTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA570.11168586879849518No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA570.11168586879849518No Hit
NNTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA560.10972646759150405No Hit
NNTGTTTGNTCNCCACGCTTTCGAGCCTCNGCGTCAGTTATCGTCCAGTA560.10972646759150405No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA550.1077670663845129No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT540.10580766517752176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)