FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55660
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA12052.1649299317283504No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA11402.0481494789795183No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5010.900107797340999No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA4710.846209126841538No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA4710.846209126841538No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA4660.8372260150916278No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA4540.8156665468918434No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTCAGTCCCTGCTTCGTG4500.8084800574919152No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA4110.7384117858426159No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA4040.7258354293927416No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA4010.7204455623427956No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA3950.7096658282429034No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA3040.5461731943945383No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA3010.5407833273445922No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2780.49946101329500536No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA2690.4832914121451671No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2550.4581386992454186No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA2540.4563420768954366No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2430.43657923104563423No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2200.3952569169960474No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA2120.3808839381961912No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1940.3485447358965145No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1940.3485447358965145No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1880.33776500179662233No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNNAGCA1850.33237513474667624No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1710.3072224218469278No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGNTAAAGCCCAGCA1710.3072224218469278No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGNGTCAGTTAAAGCNNAGCA1690.30362917714696375No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1670.3000359324469996No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1630.2928494430470715No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTCAGTCCCTGCNTCGTG1600.2874595759971254No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTCAGTCCCTGCNNCGTG1590.28566295364714334No Hit
CCTGTTTGCTACCNACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1570.2820697089471793No Hit
CCTGTTTGCTACCNACGCTTTCGTGCNTCAGNGTNAGTTAANGCNCAGCA1560.2802730865971973No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA1540.2766798418972332No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA1510.27128997484728706No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA1500.26949335249730505No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1500.26949335249730505No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA1490.26769673014732304No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1470.26410348544735895No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTNAGCA1460.26230686309737694No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1440.25871361839741286No Hit
CCTGTTTGCNACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1440.25871361839741286No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1430.25691699604743085No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTNAGCGTCAGTTAAAGCNCAGCA1420.25512037369744883No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCCAGAA1420.25512037369744883No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1390.24973050664750268No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1390.24973050664750268No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1380.24793388429752067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1380.24793388429752067No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1360.2443406395975566No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA1350.24254401724757454No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1350.24254401724757454No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1330.2389507725476105No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1320.23715415019762848No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1310.2353575278476464No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA1300.23356090549766442No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1280.22996766079770034No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA1250.22457779374775422No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA1230.22098454904779016No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1220.2191879266978081No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTCAGTCCCTGCTNCGTG1200.21559468199784404No Hit
CCTGTTTGCTACCCACGCTTTNGTGCCTCAGCGTCAGTTAAAGCCCAGCA1200.21559468199784404No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC1140.20481494789795185No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1140.20481494789795185No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1130.20301832554796984No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTNAGTCCCTGCTTCGTG1120.20122170319798777No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1110.19942508084800575No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA1110.19942508084800575No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCTGTCCTGGTCTGGTA1090.1958318361480417No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1080.19403521379805966No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCNAGAA1080.19403521379805966No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNNAGAA1030.18505210204814948No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA1030.18505210204814948No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA1020.18325547969816744No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT990.17786561264822134No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA980.1760689902982393No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA980.1760689902982393No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGTA980.1760689902982393No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA980.1760689902982393No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNNAGTG970.17427236794825726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA950.1706791232482932No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA930.16708587854832913No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA930.16708587854832913No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG880.15810276679841898No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA880.15810276679841898No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCNAGCA870.15630614444843693No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGTA850.15271289974847288No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNNAGCA840.15091627739849084No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA840.15091627739849084No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA830.1491196550485088No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA820.14732303269852676No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA820.14732303269852676No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA820.14732303269852676No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNNAGTA820.14732303269852676No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA810.14552641034854474No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA810.14552641034854474No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA800.1437297879985627No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA800.1437297879985627No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA800.1437297879985627No Hit
CCTGTTTGATACCNACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA780.14013654329859865No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCNAGTA770.1383399209486166No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA770.1383399209486166No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGTA770.1383399209486166No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA770.1383399209486166No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA760.13654329859863457No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT750.13474667624865252No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA750.13474667624865252No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA730.13115343154868847No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA720.12935680919870643No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA720.12935680919870643No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA710.12756018684872442No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA700.12576356449874238No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA700.12576356449874238No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA700.12576356449874238No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTNCAGAA680.1221703197987783No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG680.1221703197987783No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNNAGAA680.1221703197987783No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA670.12037369744879627No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA660.11857707509881424No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGNTACAGTTTAGCA660.11857707509881424No Hit
CCTGTTTGATACCNACACTTTCGAGCNTGAANGTNAGTTACNGTNTAGCA660.11857707509881424No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCNAGAA650.11678045274883221No Hit
CCTGTTNGATCCCCGCACTTTCGTGCATCAGCGTCAGTCCCTGCTTCGTG650.11678045274883221No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTNNAGAA640.11498383039885017No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTG640.11498383039885017No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA640.11498383039885017No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT630.11318720804886812No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG630.11318720804886812No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCNAGAA630.11318720804886812No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTNCAGAA620.11139058569888609No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA620.11139058569888609No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT620.11139058569888609No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTNAGTG610.10959396334890405No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA610.10959396334890405No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA610.10959396334890405No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT600.10779734099892202No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA600.10779734099892202No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA600.10779734099892202No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTNAGNCCCTGCTTCGTG590.10600071864893998No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA580.10420409629895795No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA580.10420409629895795No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA570.10240747394897592No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTNNAGAA570.10240747394897592No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTNNAGAA560.10061085159899388No Hit
CCTGTTTGATCCCNGCACTTTCGTGCATCAGCGTCAGTCCCTGCNTCGTG560.10061085159899388No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA560.10061085159899388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)