FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52496
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG21674.127933556842426No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12782.4344711978055473No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG8081.5391648887534288No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG7391.4077263029564158No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG6851.3048613227674488No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6551.2477141115513564No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA6281.1962816214568728No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG5361.0210301737275223No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG5301.0096007314843036No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA4930.939119170984456No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA4600.876257238646754No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA4410.8400640048765621No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA4280.8153002133495886No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3520.6705272782688205No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGNTGCGCTCCAGTG3420.6514782078634562No Hit
CCTGTTCGATACCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCAGTG3170.6038555318500457No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA3150.6000457177689729No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA3080.586711368485218No Hit
CCTGTTCGATACCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG3070.5848064614446815No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2890.5505181347150259No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG2770.5276592502285888No Hit
CCTGTTCGATACCCGCACTTTNGAGCTTCAGCGTCAGTTGCGCTCCAGTG2670.5086101798232245No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTNAGCGTCAGTTGCGCTNCAGTG2630.5009905516610789No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2620.4990856446205425No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA2510.4781316671746419No Hit
CCTGTTNGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG2500.4762267601341055No Hit
CCTGTTCGANACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG2490.474321853093569No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2390.4552727826882048No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGNGTCAGTTGCGCTNNAGTG2370.451462968607132No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2290.4362237122828406No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA2250.42860408412069495No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCNAGAA2140.4076501066747943No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA2140.4076501066747943No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG2040.38860103626943004No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1850.35240780249923803No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG1850.35240780249923803No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1780.3390734532154831No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1710.3257391039317281No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1690.3219292898506553No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNNAGAA1670.31811947576958244No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1660.316214568729046No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG1650.3143096616885096No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1640.3124047546479732No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1640.3124047546479732No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA1610.3066900335263639No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1570.2990704053642182No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1540.293355684242609No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1540.293355684242609No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1520.28954587016153616No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1490.28383114903992684No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1480.2819262419993904No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGAA1470.280021334958854No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGAA1470.280021334958854No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1460.2781164279183176No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTNAGTTACAGTCCAGAA1430.27240170679670833No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG1430.27240170679670833No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGCA1400.2666869856750991No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTTACGGTCCAGCA1310.24954282231027125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA1270.24192319414812558No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG1260.24001828710758913No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1200.22858884486437062No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1170.22287412374276133No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGCA1160.22096921670222494No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA1140.2171594026211521No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1120.21334958854007927No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA1120.21334958854007927No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGAA1090.20763486741846998No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1040.19811033221578786No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTCAGCGTNAGNTGCGCTCCAGTG1040.19811033221578786No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA1030.19620542517525147No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1020.19430051813471502No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA1020.19430051813471502No Hit
CCTGTTCGATTCCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG970.18477598293203293No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA950.18096616885096006No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA910.1733465406888144No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA910.1733465406888144No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG830.158107284364523No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA820.1562023773239866No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGCA810.15429747028345017No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA800.15239256324291375No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGCA790.15048765620237733No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTNAGNTACAGTCCAGAA780.14858274916184092No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA780.14858274916184092No Hit
CCTGTTNGATTCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG770.1466778421213045No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA770.1466778421213045No Hit
CCTGTTNGATTCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG770.1466778421213045No Hit
CCTGTTCGATTCCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCAGTG760.14477293508076808No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA760.14477293508076808No Hit
CCTGTTCGATTCCCGCACTTTNGAGCTTCAGCGTCAGTTGCGCTCCAGTG750.14286802804023163No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA750.14286802804023163No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTNAGCGTCAGTTGCGCTNCAGTG750.14286802804023163No Hit
CCTGTTCGANTCCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG740.1409631209996952No Hit
CCTGTTCGATTCCCGCACTTTCGAGCTTCAGNGTCAGTTGCGCTNNAGTG740.1409631209996952No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG730.1390582139591588No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA730.1390582139591588No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA720.13715330691862238No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTNAGTG720.13715330691862238No Hit
CCCGTTCGCTACCNTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA720.13715330691862238No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA710.13524839987808596No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA710.13524839987808596No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNNAGTG710.13524839987808596No Hit
CCCGTTCGCTACCCTAGCTTTNGAGCCTCAGCGTCAGTTACAGTCCAGAA710.13524839987808596No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA710.13524839987808596No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA710.13524839987808596No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG700.13334349283754954No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA700.13334349283754954No Hit
CCCGTTCGCNACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA690.13143858579701312No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA690.13143858579701312No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA680.1295336787564767No Hit
CCCGTTCGCTACCNTAGCTTTCGAGCNTCAGNGTNAGTTACNGTNCAGAA680.1295336787564767No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA680.1295336787564767No Hit
CNTGTTCGATTCCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG680.1295336787564767No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA670.12762877171594028No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA660.12572386467540386No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTGTCGTNNAGAA660.12572386467540386No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG650.1238189576348674No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTNCAGCA650.1238189576348674No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA650.1238189576348674No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA640.12191405059433098No Hit
CNCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTNAGTTACAGTCCAGAA640.12191405059433098No Hit
CCCGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA620.11810423651325815No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGNGTCAGTTACAGTNNAGAA620.11810423651325815No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCNAGTG620.11810423651325815No Hit
CCCGTTNGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA620.11810423651325815No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA610.11619932947272173No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTNAGCGTCAGTTACAGTNCAGAA610.11619932947272173No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTNNAGCA610.11619932947272173No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA600.11429442243218531No Hit
CCCGTTNGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA590.11238951539164889No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG580.11048460835111247No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA560.10667479427003963No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTGTCGTCCAGAA560.10667479427003963No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA540.10286498018896677No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG540.10286498018896677No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA530.10096007314843035No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTGTCGTNCAGAA530.10096007314843035No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA530.10096007314843035No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA530.10096007314843035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)