FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38157
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA15654.101475482873392No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA6081.5934166732185444No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA5801.520035642215059No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA5671.4859658778205835No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA5211.3654113268862855No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA3951.0351966873706004No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA3520.9225043897581047No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3150.8255365987892129No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA3020.7914668343947375No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT3010.7888460832874702No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2900.7600178211075295No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2580.6761537856749744No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2280.5975312524569542No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA2070.5424954792043399No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2050.5372539769898053No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1990.5215294703462012No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA1980.5189087192389339No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1950.5110464659171319No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1850.4848389548444584No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1790.46911444820085435No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1780.46649369709358707No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1750.458631443771785No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1740.45601069266451766No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1680.4402861860209136No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1670.43766543491364623No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1670.43766543491364623No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1670.43766543491364623No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1600.4193201771627748No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1550.4062164216264381No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1480.38787116387556675No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA1430.37476740833923006No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNNGGTA1400.366905155017428No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGCA1380.3616636528028933No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA1380.3616636528028933No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA1360.35642215058835863No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCNGGTA1360.35642215058835863No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA1320.3459391461592892No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA1320.3459391461592892No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGCA1300.34069764394475455No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1230.3223523861938832No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT1170.30662787955027915No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGCA1160.30400712844301175No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT1130.29614487512120974No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT1110.29090337290667506No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1040.27255811515580364No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA1030.2699373640485363No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA1000.2620751107267343No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA980.2568336085121996No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT920.24110910186859552No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA880.23062609743952617No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA870.22800534633225883No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA870.22800534633225883No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCACCGTCAGTTGTCGTCCAGCA870.22800534633225883No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA870.22800534633225883No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA830.21752234190318948No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA810.21228083968865477No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA800.2096600885813874No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA790.2070393374741201No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA790.2070393374741201No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA790.2070393374741201No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA760.19917708415231805No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGTTACAGTCCAGTT760.19917708415231805No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA760.19917708415231805No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA750.1965563330450507No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA730.191314830830516No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNACCGTCAGTTGTCGTNCAGCA710.18607332861598133No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA700.183452577508714No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA690.18083182640144665No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA690.18083182640144665No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA690.18083182640144665No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCACNGTNAGTTGTNGTNCAGCA680.17821107529417932No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA670.17559032418691195No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA670.17559032418691195No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNNAGTA670.17559032418691195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA670.17559032418691195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA660.1729695730796446No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGNTGCGCTCCGGTA650.17034882197237727No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA650.17034882197237727No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA640.16772807086510994No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA630.1651073197578426No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA600.15724506643604055No Hit
CCTGTTCGATACCNGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA590.15462431532877322No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA580.15200356422150588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAAAAGCCCAGCA580.15200356422150588No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGNTGTCGTCCAGCA560.1467620620069712No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA550.14414131089970386No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA550.14414131089970386No Hit
CCTGTTCGATACCCGCGCTTTNGAGCCTCAGCGTCAGTTGCGCTCCGGTA550.14414131089970386No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNNAGTT550.14414131089970386No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA540.1415205597924365No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA540.1415205597924365No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCNAGTT530.13889980868516916No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCNAGTA530.13889980868516916No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA530.13889980868516916No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGCAGTCTAGTT530.13889980868516916No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA520.13627905757790182No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA520.13627905757790182No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA520.13627905757790182No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA500.13103755536336714No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA480.12579605314883247No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA480.12579605314883247No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA480.12579605314883247No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACCGTNNAGTA480.12579605314883247No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA470.12317530204156511No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACCGTCCAGTA470.12317530204156511No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA470.12317530204156511No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA460.12055455093429776No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA460.12055455093429776No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA460.12055455093429776No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA460.12055455093429776No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNCAGTT460.12055455093429776No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA460.12055455093429776No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGNGTCAGTTGCGCTNNGGTA450.11793379982703042No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA450.11793379982703042No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNNAGGA450.11793379982703042No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA440.11531304871976308No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTNAGCGTCAGTTGCGCTNCGGTA440.11531304871976308No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTAAAAGCNNAGCA440.11531304871976308No Hit
CCTGTTCGANACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA440.11531304871976308No Hit
CCTGTTCGATACCNGCGCTTTCGAGCNTCAGNGTNAGTTGCNCTNCGGTA430.11269229761249573No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA430.11269229761249573No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA430.11269229761249573No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA430.11269229761249573No Hit
CNTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA410.10745079539796105No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA410.10745079539796105No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG400.1048300442906937No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA400.1048300442906937No Hit
CCTGTTTGCTCCCCACACTTTNGCGCCTCAGCGTCAGTTACAGTCCAGTT400.1048300442906937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)