FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15170
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA6314.159525379037574No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA5773.803559657218194No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2941.938035596572182No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA2501.6479894528675016No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNNAGTA2331.5359261700725115No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA2211.4568226763348715No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA2201.4502307185234016No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCNAGTA2191.4436387607119314No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2091.3777191825972315No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2031.3381674357284112No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA1811.1931443638760713No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1601.054713249835201No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1521.001977587343441No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAATCCAGTA1010.6657877389584707No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1010.6657877389584707No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT990.6526038233355307No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA940.6196440342781806No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNATNCAGTA930.6130520764667106No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA920.6064601186552405No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA910.5998681608437706No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA880.5800922874093606No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA860.5669083717864206No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA840.5537244561634805No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA830.5471324983520105No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAATNCAGTA800.5273566249176005No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA780.5141727092946605No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA770.5075807514831905No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAATNNAGTA760.5009887936717204No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCTAGTG740.4878048780487805No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA740.4878048780487805No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA730.48121292023731044No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA730.48121292023731044No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAATCCAGTA720.4746209624258405No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA710.46802900461437047No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA680.44825313117996046No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA680.44825313117996046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA670.4416611733684904No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA660.4350692155570204No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA660.4350692155570204No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA660.4350692155570204No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA640.4218852999340804No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA630.41529334212261043No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA630.41529334212261043No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA620.4087013843111404No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA620.4087013843111404No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA610.40210942649967035No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA590.3889255108767304No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA590.3889255108767304No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA550.3625576796308504No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT480.31641397495056034No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA470.3098220171390903No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA450.2966381015161503No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA450.2966381015161503No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTT400.26367831245880025No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA390.25708635464733026No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTA390.25708635464733026No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG390.25708635464733026No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA380.2504943968358602No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG380.2504943968358602No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA370.24390243902439024No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTNNAGTG360.23731048121292025No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA350.23071852340145024No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA330.2175346077785102No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT330.2175346077785102No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA320.2109426499670402No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA320.2109426499670402No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA310.2043506921555702No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT300.1977587343441002No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA300.1977587343441002No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA300.1977587343441002No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTNCAGAA290.19116677653263017No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTT290.19116677653263017No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA290.19116677653263017No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA280.1845748187211602No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA280.1845748187211602No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA280.1845748187211602No Hit
CCTGTTTGCTACCCATGCTTTCGTGCTTCAGCGTCAGTTAAAGCCCAGTA280.1845748187211602No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTTCTGTCCAGAA270.17798286090969018No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA270.17798286090969018No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA270.17798286090969018No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA270.17798286090969018No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCNAGTG270.17798286090969018No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACCGTNNAGTA270.17798286090969018No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTNNAGAA260.17139090309822017No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA260.17139090309822017No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA260.17139090309822017No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCNAGAA260.17139090309822017No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA260.17139090309822017No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTA250.16479894528675015No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA250.16479894528675015No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA240.15820698747528017No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA240.15820698747528017No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA230.15161502966381013No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA230.15161502966381013No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA230.15161502966381013No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTNTAGTG220.14502307185234015No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNNAGTT220.14502307185234015No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA210.13843111404087013No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA200.13183915622940012No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA200.13183915622940012No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA200.13183915622940012No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA200.13183915622940012No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA200.13183915622940012No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA200.13183915622940012No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA200.13183915622940012No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGTA190.1252471984179301No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACCGTCCAGTA190.1252471984179301No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA180.11865524060646013No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA180.11865524060646013No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA180.11865524060646013No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA180.11865524060646013No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA170.11206328279499012No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT170.11206328279499012No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA170.11206328279499012No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA170.11206328279499012No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGANCAGAG170.11206328279499012No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA170.11206328279499012No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG170.11206328279499012No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA170.11206328279499012No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTNNAGTG160.1054713249835201No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA160.1054713249835201No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCNAGTT160.1054713249835201No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCNAGTG160.1054713249835201No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA160.1054713249835201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)